Free tool

Follow the funding.

Every study behind a Caveat check, searchable, with who paid for it named out loud. These are our own verified appraisals, not a guess from a database. Independent, no industry money.

414studies appraised
80checks
38%had industry money (where funding was disclosed)

Independent 212 · Industry 94 · Mixed 37 · Unknown 41 · N/A 30

Showing 414 of 414 appraised studies across 80 checks

  • DietAlcohol Misleading
    • Independent

      Zhao/Stockwell 2023, bias-corrected meta-analysis

      Systematic review and meta-analysis of cohorts · 107 studies; ~4.8M people; ~425,564 deaths

      Canadian Centre on Substance Use and Addiction subcontract on a Health Canada grant; no alcohol-industry money. Stockwell disclosed expert-witness fees.

      Limits: Observational; relies on correcting abstainer bias, not on randomized trials.

    • Independent

      Lankester 2021, Mendelian randomization (UK Biobank)

      Mendelian randomization · ~337,484 in MR analysis

      US NIH NHLBI and NIDDK grants. Co-author Ingelsson disclosed prior GlaxoSmithKline employment (pharma, not alcohol).

      Limits: MR assumes no pleiotropy; ADH1B variant rare in some populations; some estimates attenuate in multivariable models.

    • Independent

      GBD 2016 Alcohol Collaborators (Lancet 2018)

      Comparative risk assessment · 195 countries; 694 data sources; 592 risk studies

      Gates Foundation / academic (IHME, University of Washington); no industry funding.

      Limits: Modeled relative risks pooled across heterogeneous sources.

    • Independent

      WHO 2023 statement / Lancet Public Health

      Position statement synthesizing evidence · Policy statement

      World Health Organization; no industry funding.

      Limits: Statement, not primary data; rests on IARC carcinogen classification.

    • Industry-funded

      NIH MACH15 cancellation (STAT 2018)

      News report on a cancelled RCT · Trial planned ~7,800 participants (terminated)

      ~$100M largely from liquor companies via the NIH Foundation; cited as evidence of conflict, not of an alcohol effect.

      Limits: Trial never ran; design judged too short to detect cancer harms.

    • Independent

      Mirick 2002 (breast cancer)

      Population-based case-control · 813 cases, 793 controls, ages 20-74

      Fred Hutchinson Cancer Research Center; abstract states no funder, so 'independent' is an inference.

      Limits: Single case-control design subject to recall bias; one of few dedicated studies.

    • Independent

      Flarend 2001 (dermal absorption)

      Human Al-26 isotope tracer (preliminary) · 2 healthy adults

      Academic tracer study; PubMed tags US Gov't Non-PHS / Non-US Gov't, no industry money.

      Limits: Tiny n=2 preliminary design; absorption ~0.012% of dose.

    • Industry-funded

      de Ligt 2022 (dermal absorption)

      Human Al-26 microtracer with IV reference · 12 healthy women

      100% funded by Cosmetics Europe; five authors employed by cosmetics companies.

      Limits: Small n=12; favourable to industry but directionally agrees with Flarend (~0.0005%).

    • Independent

      Alzheimer's Drug Discovery Foundation review

      Authority evidence review · Cites antacid meta-analysis >6,000 people

      Non-profit research foundation; no antiperspirant-industry tie.

      Limits: Review, not primary data; antacid/occupational exposures, not antiperspirant cohort.

    • Not applicable

      American Cancer Society position

      Authority position statement · na

      Major non-profit; no relevant commercial conflict.

      Limits: Summary statement, not a primary study.

    • Independent

      WHO/IARC/JECFA joint aspartame statement (2023)

      Regulatory hazard + risk assessment · N/A

      WHO/FAO expert bodies; no commercial sponsor.

      Limits: Hazard tier (2B) is widely misread as real-world risk; based on limited evidence.

    • Independent

      WHO non-sugar-sweetener guideline (2023)

      Clinical guideline (conditional) · N/A

      Publicly funded WHO process.

      Limits: Underlying evidence low-certainty; a benefit/risk message, not a toxicity finding.

    • Mixed funding

      Suez et al., Cell (2022)

      RCT + gnotobiotic mouse transplant · 120 adults, 2 weeks

      Weizmann lab; senior authors Segal and Elinav co-founded personalized-nutrition firm DayTwo, a conflict on the gut-harm narrative.

      Limits: Short; surrogate markers; large person-to-person variation; aspartame/stevia null.

    • Independent

      Debras et al., PLOS Medicine (2022)

      Observational cohort · 102,865 adults

      Publicly funded (Inserm/Sorbonne Paris Nord).

      Limits: Self-reported intake; residual confounding and reverse causation; small effect sizes.

    • Independent

      Serrano et al., Microbiome (2021)

      Null human + mouse trial · Humans 2 wk, mice 10 wk

      Academic; the neutral counter-evidence.

      Limits: Saccharin only; human arm at maximum acceptable intake, not above it.

    • Industry-funded

      Chandrasekhar 2012, stress/cortisol RCT

      Double-blind placebo-controlled RCT · n=64, 60 days

      Used branded KSM-66 extract supplied by Ixoreal Biomed; paper prints nil-COI, a foundational KSM-66 marketing trial.

      Limits: Small, short, single-site; commercial extract.

    • Industry-funded

      Lopresti 2019, stress/cortisol/testosterone RCT

      Double-blind placebo-controlled RCT · n=60, 60 days

      Funded by Arjuna Natural Ltd, which supplied the Shoden extract.

      Limits: Small; testosterone non-significant between groups.

    • Mixed funding

      BJPsych Open 2025 meta-analysis

      Systematic review and meta-analysis · 15 RCTs, n=873

      Pools predominantly small industry-linked branded-extract trials.

      Limits: Inherits source-trial bias; no QoL improvement.

    • Independent

      Cheah 2021 sleep meta-analysis

      Systematic review and meta-analysis · 5 RCTs, n=400

      Review unfunded, no competing interests; flags one included trial as manufacturer-sponsored.

      Limits: All India; I2=62%; moderate quality.

    • Industry-funded

      Wankhede 2015 strength/testosterone RCT

      Double-blind placebo-controlled RCT · n=57, 8 weeks

      Used branded KSM-66 (Ixoreal); most-cited male-vitality result.

      Limits: Both arms did resistance training: effect confounded.

    • Independent

      Mandsager et al., JAMA Network Open, 2018

      Retrospective observational cohort · n=122,007; median 8.4-yr follow-up; 13,637 deaths

      Conflicts of interest: "None reported." Single academic center (Cleveland Clinic); no industry sponsor. Authors explicitly state the association "does not prove causation."

      Limits: Observational; cannot rule out reverse causation/preselection (authors say so). Population referred for treadmill testing, not a general-population sample.

    • Independent

      Kodama et al., JAMA, 2009 (meta-analysis)

      Meta-analysis of 33 observational cohorts · ~102,980 participants; 6,910 deaths (all-cause analysis)

      Listed as "Research Support, Non-U.S. Gov't." No commercial product sponsor.

      Limits: Pools observational cohorts only, establishes a consistent dose-response association, not causation.

    • Independent

      Ross et al., AHA Scientific Statement, Circulation, 2016

      Scientific statement / expert consensus · Synthesis of observational evidence (no single sample)

      American Heart Association statement; indexed record lists two VA grants (I01 RX000344, IK6 RX002477). No commercial product sponsor.

      Limits: Synthesizes observational data; explicitly frames fitness as a predictor/vital sign, not a proven causal intervention.

    • Mixed funding

      Lee et al., Aerobics Center Longitudinal Study, Circulation, 2011

      Prospective observational cohort · n=14,345 men; ~11.4-yr follow-up; 914 all-cause / 300 CVD deaths

      NIH grants (AG06945, HL62508, DK088195) PLUS "an unrestricted research grant from The Coca-Cola Company." Authors declared no conflicts. The Coca-Cola tie is flagged given the company's documented interest in framing inactivity, rather than diet, as the driver of poor health, an interest this study's pro-fitness conclusion happens to serve.

      Limits: Observational; men only; referral/treadmill-tested population; the change-in-fitness inference rests on this single-sex cohort. Weakest of the anchor studies, should not carry the "improving fitness" claim alone.

    • Independent

      Kokkinos et al., JACC, 2023 (VA Exercise Testing cohort)

      Retrospective observational cohort · n=93,060 veterans; two treadmill tests ~5.8 yr apart; median 6.3-yr follow-up

      Veterans Affairs / academic. Authors reported "no relationships relevant to the contents of this paper to disclose." No commercial sponsor identified.

      Limits: Observational; veteran population skews male/middle-aged; a ≥2-MET fitness decline tracked with 69–74% higher mortality, but change-in-fitness associations remain susceptible to reverse causation.

    • Independent

      Kjaergaard et al., J Clin Endocrinol Metab, 2025 (Mendelian randomization)

      2-sample Mendelian randomization (genetic causal inference) · VO₂max instrument ~70,000 (UK Biobank); longevity ~1,012,240 parents

      Novo Nordisk Foundation, NIH NIDDK (R01 DK124258, R01 DK129850), Boston Children's Hospital endowment. "The authors have nothing to disclose." Transparency note: the Novo Nordisk Foundation is a pharma-linked philanthropy; the null finding here does not favor any product, so the conflict risk is low.

      Limits: VO₂max instrument uses *estimated* fitness (submaximal test); some GWAS sample overlap; parental lifespan is a noisy longevity proxy. The null is not definitive, but the same instruments detected expected upstream effects, so it is a genuine signal, not a dead instrument.

    • Independent

      Dibben et al., Cochrane Review, 2021 (exercise-based cardiac rehab)

      Cochrane systematic review / meta-analysis of RCTs · 85 randomized trials; 23,430 coronary heart disease patients

      Cochrane Collaboration review; standard Cochrane independence/COI policy.

      Limits: All-cause mortality non-significant (RR 0.87 short-term, 0.91 long-term; CIs cross 1.0; moderate certainty, downgraded for imprecision). Population is coronary-disease patients, not healthy adults, transfers imperfectly to the general claim.

    • Independent

      Ballin & Nordström, J Intern Med, 2021 (critical review of RCTs)

      Critical review of randomized-trial evidence · Narrative synthesis of long-term exercise RCTs

      Academic (Umeå University); no commercial sponsor identified.

      Limits: Narrative, not a pooled meta-analysis. Concludes RCT evidence "shows that exercise does not prevent premature mortality or CVD", an active negative finding, weighed alongside the structural reasons large mortality-powered exercise trials are rarely run.

    • Independent

      EMBO Reports SENS critique (Warner et al.)

      Expert consensus commentary, peer-reviewed · ~28 co-signatory biogerontologists

      University-based academics; no industry sponsorship indicated.

      Limits: Opinion/commentary, not an empirical study; full abstract not rendered on PubMed, substance corroborated via tertiary sources.

    • Independent

      MIT Technology Review SENS Challenge

      Adjudicated scientific debate · 5 submissions; independent expert judges

      Run with the Methuselah Foundation; judging panel independent.

      Limits: Cited URL is the Estep dissent page, not the judges' verdict; the partial-prize figure is not verifiable there.

    • Industry-funded

      LEV Foundation Robust Mouse Rejuvenation Study 1 (design)

      Preclinical combination-therapy mouse study · 1,000 mice (500M/500F)

      Claimant's own foundation; reliable for design, interested party on results.

      Limits: Design page only; no outcomes reported.

    • Independent

      Independent analysis of RMR1 survival data (Gowing Life)

      Independent secondary analysis · Analysis of the 1,000-mouse dataset

      Third-party science blog, not selling the therapies.

      Limits: Based on preliminary, non-peer-reviewed data.

    • Independent

      Liu D et al., berberine on metabolic syndrome components, meta-analysis, Frontiers in Pharmacology 2025

      Systematic review & meta-analysis of placebo-controlled RCTs · Multiple placebo-controlled RCTs

      Paper declares no commercial or financial conflict of interest.

      Limits: Pooled trials are small, heterogeneous and predominantly Chinese; BMI effect borderline.

    • Funding unknown

      Xie et al., glucose-lowering effect of berberine in type 2 diabetes, meta-analysis, Frontiers in Pharmacology 2022

      Systematic review & meta-analysis (37 RCTs) · 37 RCTs, 3,048 patients

      Academic meta-analysis; funding not clearly stated.

      Limits: Authors note studies mostly China-based and of moderate quality (poor allocation concealment/blinding).

    • Mixed funding

      Yin J, Xing H, Ye J, berberine in type 2 diabetes, Metabolism 2008

      Randomized controlled trial (small cohorts) · n=36 (Study A) + n=48 (Study B)

      Supported by Xinhua Hospital plus partial NIH grant support; no pharmaceutical sponsor. Conducted in China.

      Limits: Small; ~34.5% GI side effects across studies; weight outcomes for Study A not reported in the abstract.

    • Industry-funded

      Rubino D et al., STEP 4, semaglutide weight-loss maintenance, JAMA 2021

      Randomized controlled trial (comparator benchmark) · n=803

      Sponsored by Novo Nordisk; authors include company employees and a shareholder. Favourable result.

      Limits: Cited only as the semaglutide benchmark, not as berberine evidence.

    • Industry-funded

      Garvey WT et al., STEP 5, two-year semaglutide, Nature Medicine 2022

      Randomized controlled trial (comparator benchmark) · n=304

      Novo Nordisk funded and ran the trial. Favourable result.

      Limits: Industry-run; cited only as the comparator standard.

    • Independent

      Poulain & Herm, Okinawa demographic trends, J Intern Med 2024

      Demographic analysis · Okinawa population & centenarian counts, 1975-2021

      Academic; Poulain co-originated the Blue Zone concept, so biased toward defending it, yet reports erosion.

      Limits: Confirms pre-1940 advantage but post-WWII mortality now higher than mainland Japan; authors urge age validation.

    • Independent

      Poulain & Herm, 'Blue Zone, a Demographic Concept and Beyond', Am J Lifestyle Med 2025

      Narrative review · NA (review of regions)

      Authors are concept originators (pro-Blue-Zones intellectual COI), strengthening their causality concession.

      Limits: A defense, yet concedes diet's causal role is unproven and the diet is not homogeneous or stable over time.

    • Industry-funded

      Buettner & Skemp, 'Blue Zones: Lessons', Am J Lifestyle Med 2016

      Narrative review (claimant) · 5 regions; diet figure from 154 surveys

      Authored by the founder of Blue Zones LLC; direct commercial conflict.

      Limits: Self-authored statement of the claim; the 95%-plant and 10x-US figures are not independently verified.

    • Independent

      Tan et al., plant-based diet & mortality meta-analysis, Front Nutr 2024

      Systematic review / meta-analysis · 14 cohort articles

      Academic; no industry sponsor identified.

      Limits: Healthy plant index RR 0.85 (0.80-0.90); unhealthy plant index RR 1.18 raises mortality. Observational; modest effect.

    • Independent

      Holt-Lunstad et al., social relationships & mortality, PLoS Med 2010

      Meta-analysis · 148 studies; ~308,849 people

      Academic; no commercial conflict.

      Limits: OR 1.50 (1.42-1.59) for survival; 'comparable to well-established risk factors.' Observational.

  • PeptidesBPC-157 Unproven
    • Funding unknown

      Vasireddi et al., HSS Journal 2025, systematic review

      Systematic review (35 preclinical + 1 uncontrolled human case series) · 36 studies; sole human data = 12-patient uncontrolled knee-injection series

      No funding or conflicts disclosed in the accessible record.

      Limits: Underlying evidence graded Level IV/V; single group dominates the literature; no human RCT exists.

    • Independent

      He et al., Frontiers in Pharmacology 2022, animal PK

      Preclinical pharmacokinetics (rats and dogs; zero humans) · Sprague-Dawley rats and beagle dogs; n humans = 0

      Funded by China's National Natural Science Foundation and Shaanxi provincial programs; authors declared no commercial/financial ties.

      Limits: Animal-only; very short half-life (<30 min); human PK and dosing entirely unknown.

    • Industry-funded

      Novo Nordisk citizen petition (FDA-2024-P-4937) quoting FDA's BPC-157 Category 2 rationale

      Industry-authored regulatory filing quoting FDA finding · n/a (regulatory)

      Authored by Novo Nordisk (commercial interest), not the FDA; it quotes FDA's actual BPC-157 immunogenicity/impurity language verbatim.

      Limits: Petition is chiefly about semaglutide; used only for the verbatim FDA BPC-157 safety quote, not as a primary FDA document.

    • Industry-funded

      NCT02637284, Phase 1 oral BPC-157 safety trial (PharmaCotherapia)

      Phase 1 human safety/PK trial, results never posted · ~42 healthy volunteers (results never released)

      Sponsor PharmaCotherapia, tied to patent-holder P. Sikiric; classic sponsor/COI red flag.

      Limits: CT.gov status is 'Unknown' (last active 2015); no human results ever posted; 'withdrawn before review' detail is STAT-sourced.

    • Independent

      STAT News investigation, Feb 2026

      Independent investigative journalism · n/a

      Independent newsroom; corroborates COI, evidence gap and grey-market purity concerns.

      Limits: Journalism, not primary data; cancer/angiogenesis concern is theoretical and disputed by Sikiric's group.

    • Independent

      Belsky et al., DunedinPACE (eLife)

      Biomarker development/validation cohort · N=1,037 birth cohort, ages 26-45, 4 timepoints

      Academic (Duke/Columbia/King's College London); NIH and related public funding. Authors developed the algorithm.

      Limits: Population-level reliability (ICC ~0.96); authors state surrogate-endpoint status is not yet established. Validates the clock for research, not for certifying one person's response.

    • Independent

      Waziry/Ryan/Belsky, CALERIE caloric restriction RCT (Nature Aging)

      Randomized controlled trial (secondary methylation analysis) · 220 randomized; 197 with methylation data; 2 years; 25% calorie restriction vs ad libitum

      NIH/NIA-funded CALERIE consortium; not industry-funded.

      Limits: Only ~2-3% group-level DunedinPACE slowing; PhenoAge/GrimAge unchanged; no disease/mortality outcomes yet. Tests calorie restriction, not Blueprint.

    • Independent

      Higgins-Chen & Levine, clock reliability (Nature Aging)

      Methods/reliability study · Multiple methylation datasets and technical replicates (six clocks)

      Academic (Yale); NIH/NIA + NIMH public funding. Authors are clock methodologists, not product sellers.

      Limits: Shows up to ~9-year disagreement between replicate reads of the same sample; reliable tracking needed a PC retraining fix. A single n=1 reading is weak without noise control.

    • Independent

      Apsley, Etzel, Ye & Shalev, limits of clocks as personal biomarkers (Epigenomics)

      Peer-reviewed review/analysis · n/a (review)

      NIH/NSF-funded academics (Penn State; Univ. of Illinois Urbana-Champaign); no product interest.

      Limits: Concludes clocks 'fail to meet common standards for clinical utility' and individual use 'can be uninformative and potentially harmful.' Argument, not a Blueprint-specific experiment.

    • Independent

      Shalev & Apsley, plain-language explainer (The Conversation)

      Expert commentary (NIH/NSF-funded academics) · n/a

      Authors are NIH/NSF-funded academics (Penn State; Univ. of Illinois); no product interest.

      Limits: Popular-press piece; states clocks are 'not appropriate tools for individual health decisions' and best used on populations. Notes fluctuation with diet/illness/time of day. Does not itself discuss seller conflicts of interest.

    • Independent

      Borrus et al., 'When to Trust Epigenetic Clocks' (bioRxiv preprint)

      Retrospective re-analysis (preprint, not peer-reviewed) · 10 longitudinal datasets (6 interventions, 2 positive controls, 2 diet arms)

      Academic preprint (Yale Dept. of Psychiatry; NIA grants + Yale fellowships); no product-seller funding.

      Limits: Preprint, used for context only. Shows first-generation clocks throw false positives that vanish after correction; reliable signals need multiple clocks to agree.

    • Funding unknown

      Levine critique of the '31 years' math (commentary, via NMN.com)

      Expert critique / secondary reporting · n/a

      Published on a site in the longevity-supplement space (NMN.com); used only as a conduit for Morgan Levine's methodological argument, which aligns with sources 1 and 3.

      Limits: Not peer-reviewed and hosted on a commercial site; cited only for the methodological point that pace cannot be converted into 'years.'

    • Independent

      ACSH critique of the Blueprint protocol

      Independent expert critique · n/a

      American Council on Science and Health, an independent 501(c)(3) nonprofit; no stake in Blueprint.

      Limits: Opinion/analysis, not original data. Characterizes results as 'a case study' with 'no way to guarantee' causation; notes abandoned interventions.

    • Industry-funded

      Blueprint 'Speed of Aging' test product page (money trail)

      Primary claimant source / commercial listing · n=1 (self)

      Seller's own site; Johnson profits from the test and products underlying the claim.

      Limits: Confirms the $325 DNA-methylation test across 11 organ systems (conflict-of-interest evidence). Does not itself carry the headline quotes.

    • Industry-funded

      Johnson, 'I have the world's slowest speed of aging' (Blueprint blog)

      Primary claimant source · n=1 (self)

      Johnson's own commercial site; he sells the products and test behind the claim.

      Limits: Source of the verbatim claims (0.48; 'birthday every 2 years'; three-test average 0.54; #1 of 5,677). Self-reported, uncontrolled, marketing context.

    • Industry-funded

      Asadi Shahmirzadi et al., Cell Metabolism (mouse lifespan/healthspan)

      Preclinical RCT in mice (n=183) · 183 mice

      Author equity/board roles in Ponce de Leon Health; patents filed. Peer-reviewed.

      Limits: Lifespan gain modest; significant in females only. Full text not directly accessible (403).

    • Industry-funded

      Demidenko et al., Aging (the '8-year' human study)

      Retrospective, open-label, uncontrolled observational · 42 customers

      Data from Ponce de Leon customers; testing by partnered lab TruMe; co-author on company board.

      Limits: No control, no randomization, self-selected; 9-CpG surrogate clock. Cannot show causation.

    • Not applicable

      Buck Institute press release

      Institutional press release · na

      From institution employing some authors; magnitude framing only.

      Limits: Not independent evidence; states human data still needed.

    • Industry-funded

      GlobeNewswire / Ponce de Leon press release

      Corporate marketing communication · na

      Issued for the product marketer.

      Limits: Amplifies the n=42 study; not independent confirmation.

    • Mixed funding

      ABLE RCT protocol, GeroScience

      Double-blind placebo-controlled RCT protocol · 120 planned

      Academically run (Singapore/Netherlands) but study product supplied by Ponce de Leon; Kennedy on advisory board.

      Limits: Results not yet published; small (n=120), short (6 months); surrogate endpoint.

    • Independent

      Lennerz et al. 2021, the most-cited carnivore survey

      Cross-sectional online survey (uncontrolled, self-reported) · 2,029 self-selected respondents (67% male, median age 44)

      NIH grants (K23 DK119546, R03 DK123541, T32 AT004094); no industry funding. A descriptive survey, not an efficacy test.

      Limits: No control group; self-reported outcomes and lipids; heavy selection and recall bias; authors call it preliminary.

    • Not applicable

      Kirwan et al. 2022, peer-reviewed critique of the survey

      Published letter / methodological critique · N/A (methodological critique)

      Letter; no relevant funding. Establishes the survey's limitations are recognised in the peer-reviewed literature.

      Limits: Not original data; a critique. Notes echo-chamber selection, unverified lipids, 28 duplicate responses.

    • Mixed funding

      Klement & Matzat 2025, German pre/post bloods

      Non-randomized explorative mixed-methods study (uncontrolled) · 24 quantitative + 4 qualitative interviews

      No external study funding, but lead author follows an animal-based diet and received refunds from a carnivore supplement supplier (carnivoro.eu); a personal/supplier conflict, not study funding.

      Limits: No control, no randomization, variable timing, mostly self-reported diet; despite favourable bias still shows a large adverse LDL rise.

    • Independent

      Wang et al. 2016, red/processed meat and mortality

      Dose-response meta-analysis of prospective cohorts (observational) · 17 cohorts; 150,328 deaths

      National Natural Science Foundation of China (NSFC 81370966); no competing interests declared.

      Limits: Observational, residual confounding; unprocessed-red-meat associations weaker and significant only in US populations.

    • Independent

      Goedeke et al. 2024, nutrient modelling of carnivore plans

      Descriptive nutrient-composition modelling / case study · 4 modelled meal plans (no human participants)

      No external funding, no conflicts; authors include low-carb-sympathetic researchers, which strengthens the deficiency findings.

      Limits: Models hypothetical plans, not measured intakes; real-world supplementation could offset some gaps.

    • Independent

      Liao 2026, systematic review + meta-analysis (Eur J Med Res)

      Systematic review and meta-analysis · 23 studies; 1,074 non-diabetic participants; 11 countries (7 RCTs)

      Chinese government research programmes (National Key R&D; Guangdong Provincial S&T; TZI-ZJU). Authors declared no competing interests.

      Limits: Very recent; full risk-of-bias and heterogeneity appraisal not independently re-run. Pooled mean-glucose improvement is driven by prediabetic subgroups, not healthy users.

    • Industry-funded

      Berry 2020, PREDICT 1 (Nat Med)

      Large observational metabolic-phenotyping cohort · ~1,002 UK twins and unrelated adults; 100 US validation

      Supported by ZOE (Zoe Global Ltd) plus Wellcome/MRC/BHF; multiple authors are consultants to or employees of ZOE, which sells products built on PREDICT.

      Limits: Establishes between-person variability only; does not test whether flattening curves improves any health outcome.

    • Independent

      Hall 2018, 'glucotypes' (PLoS Biol)

      Small exploratory observational cohort · 57 participants (38 normoglycemic, 14 prediabetic, 5 diabetic); 30 in standardized-meal subset

      Declared 'no competing interests'; funded by NIH/NSF/Stanford. But senior author Michael Snyder co-founded January AI, a commercial CGM company, an undisclosed conflict (per source 6).

      Limits: Small, hypothesis-generating; authors call for long-term outcome studies. Documents patterns, not benefits of acting on them.

    • Independent

      Shah 2019, normative CGM profiles (J Clin Endocrinol Metab)

      Multicenter prospective observational (reference ranges) · 153 healthy non-diabetic participants, ages 7 to 80

      Leona M. and Harry B. Helmsley Charitable Trust (non-commercial). Lead author discloses Dexcom/Sanofi advisory and consultancy ties; funding source itself is independent.

      Limits: Descriptive normative data, not an intervention; establishes what 'normal' looks like, not whether changing it helps.

    • Mixed funding

      Holzer 2022, CGM in healthy adults (Sensors)

      Narrative mini-review / perspective · No primary sample

      'No external funding.' One co-author sits on the Abbott Advisory Board; others declare none.

      Limits: Narrative review, not systematic; corroborates that outcome evidence in healthy people is limited and CGM has lag/accuracy constraints.

    • Independent

      Roberts 2015, post-exercise cold immersion vs active recovery

      Randomized controlled trial (12 wk) + acute cross-over · n=21 (RCT); n=9 (acute)

      ACSM Research Foundation and Exercise & Sport Science Australia; authors declare no competing interests.

      Limits: Small; effect shown for immersion immediately after resistance training, not rest-day plunges.

    • Independent

      Bleakley 2022 Cochrane, cold immersion for muscle soreness

      Systematic review / meta-analysis · 17 trials, 366 participants

      Cochrane editorial independence; specific funding not stated.

      Limits: Low evidence quality; literature search extends only to Nov 2011; adverse events mostly unmonitored.

    • Funding unknown

      Sramek 2000, immersion at varied temperatures

      Within-subject physiological experiment · n=10 (healthy men)

      Funding not stated in record.

      Limits: Tiny sample, young healthy men; basis for the exact catecholamine/metabolic-rate magnitudes.

    • Funding unknown

      Jansky 1996, sympathetic response to cold immersion

      Controlled physiological experiment · single-digit, healthy men

      Funding not stated in record.

      Limits: Very small; found no significant dopamine rise, tempering Sramek's dopamine figure.

    • Independent

      Tabei 2024, metabolic effects of cold-activated BAT

      Systematic review / meta-analysis · 7 studies, n=85

      Deutsche Forschungsgemeinschaft (DFG) and Chilean agencies (VID, Fondecyt).

      Limits: No body-weight or fat-mass outcomes assessed; small pooled sample.

    • Independent

      Myung & Park 2025, Am J Med, independent meta-analysis (23 RCTs)

      Systematic review & meta-analysis of RCTs · 23 RCTs, 1,474 participants

      Authors reported no funding and no conflicts (triangulated; full text paywalled).

      Limits: Subgroup effect sizes for the funded-vs-independent split not available in open materials; classification of some 'independent' trials is disputed by industry.

    • Mixed funding

      Pu et al. 2023, Nutrients, meta-analysis (26 RCTs)

      Systematic review & meta-analysis of RCTs · 26 RCTs, 1,721 participants

      Review itself funded by Taipei Municipal Wanfang Hospital, no COI; but pools many industry-funded primary trials with no funding subgroup.

      Limits: Missing outcome data in 13 studies, small samples, instrument-dependent readouts.

    • Funding unknown

      Dewi et al. 2023, Cureus, meta-analysis (14 RCTs)

      Systematic review & meta-analysis of RCTs · 14 RCTs, 967 participants (492 collagen / 475 placebo)

      No competing interests declared; no explicit funding statement given.

      Limits: Pools industry-funded primary trials; short durations; wrinkle effect small.

    • Industry-funded

      Bolke et al. 2019, Nutrients, single-product RCT (Elasten)

      Randomized, placebo-controlled, blinded RCT · n=72 women (36/36), 12 weeks + 4-week follow-up

      Funded by Quiris Healthcare; three of four authors affiliated with contract lab Dermatest (fourth is a university statistician).

      Limits: Bundles co-actives (vitamin C, zinc, biotin, vitamin E), confounding attribution to collagen.

    • Industry-funded

      Q-SYMBIO RCT (Mortensen 2014, JACC Heart Fail)

      Randomized double-blind placebo-controlled trial · 420 chronic HF patients, 2-yr follow-up

      Pharma Nord, Kaneka and International CoQ10 Association; favourable sponsor-funded result, flagged.

      Limits: Small death counts, imprecise estimates; not guideline first-line.

    • Industry-funded

      Alehagen 2013 KiSel-10 baseline (Int J Cardiol)

      RCT (combination: selenium + CoQ10) · 443 elderly Swedes, 4 yr

      Pharma Nord supported; CoQ10 marketer.

      Limits: Combination in selenium-deficient elderly; CV mortality 5.9% vs 12.6% (p=0.015); CoQ10's solo effect unknown.

    • Industry-funded

      Alehagen 2015 10-yr follow-up (PLOS One)

      Long-term RCT follow-up (combination) · 443 (221 active / 222 placebo)

      Pharma Nord supported analysis costs.

      Limits: HR 0.51 (95% CI 0.36-0.74) is for the combination, not CoQ10 alone.

    • Industry-funded

      J Nutr Sci 2025 statin-myopathy meta-analysis

      Systematic review and meta-analysis · 7 RCTs, 389 patients

      Funded by Society for Applied Vitamin Research (GVF); industry-adjacent, reclassified per audit.

      Limits: Muscle-pain WMD -0.96 but I-squared = 93%; pooled estimate unreliable.

    • Independent

      Jafari 2018 umbrella review (Indian Heart J)

      Systematic review of systematic reviews · 7 reviews, 71 RCTs (~4,688)

      Academic; no industry funding (None declared).

      Limits: Rated evidence uncertain; supports adjunctive, not first-line, use; the hard mortality data lean heavily on Q-SYMBIO (Caveat analysis).

    • Independent

      Avgerinos 2018 systematic review

      Systematic review of 6 RCTs · 6 RCTs, 281 individuals

      NIH/National Institute on Aging-affiliated author (Kapogiannis); funded by NIH intramural grants. No creatine-industry funding.

      Limits: Few, small, heterogeneous trials; benefit outside memory/reasoning called 'unclear'.

    • Mixed funding

      Prokopidis 2023 memory meta-analysis

      Systematic review & meta-analysis of RCTs · 8 RCTs, 225 participants

      Paper states 'No external funds supported this work,' but author COIs are real: Forbes a former scientific advisor to a creatine company; Candow on AlzChem's (creatine maker) Scientific Advisory Board. Unfunded but conflicted.

      Limits: I-squared 66%; overall effect later shown to rest on a double-counting error and to hold only in older adults on re-analysis.

    • Not applicable

      Letter to the Editor + authors' reply (methodological critique)

      Methodological critique of Prokopidis 2023 · Re-analysis of the 8-RCT / 225-participant dataset

      Methodological commentary; no funding relevant.

      Limits: Abstract-level; specific re-analysis outcome confirmed via authors' reply rather than the letter abstract itself.

    • Independent

      Xu 2024 cognition meta-analysis (independent)

      Systematic review & meta-analysis of RCTs · 16 RCTs, 492 participants

      Funded by the National Natural Science Foundation of China; authors declare no commercial conflicts. Key independent corroboration of the memory signal.

      Limits: High heterogeneity; null for overall cognition and executive function; short-term cognitive-test outcomes only.

    • Independent

      Gordji-Nejad 2024 sleep-deprivation crossover

      Randomised double-blind crossover (acute single dose) · 15 participants

      Funded by Forschungszentrum Julich and RWTH Aachen (academic); one author disclosed unrelated industry ties (imaging firms), no creatine-industry funding.

      Limits: Very small n; single very high acute dose (0.35 g/kg) under an extreme stressor, not typical daily use.

    • Funding unknown

      Daily 2016, turmeric/curcumin for joint arthritis (meta-analysis)

      Systematic review and meta-analysis of 8 RCTs · 8 RCTs, ~1000 mg/day

      No funding/COI statement in the abstract; OA curcumin trials in this field are frequently maker-sponsored.

      Limits: Authors state trial number, sample size and methodological quality insufficient for definitive conclusions.

    • Independent

      Wai 2025, turmeric products for knee OA (network meta-analysis)

      Systematic review and network meta-analysis of RCTs · 17 RCTs (913 in primary pain analysis)

      Paper states it received no specific grant and declares no competing interests; pooled primary trials still include maker-sponsored products.

      Limits: GRADE certainty low to very low for all significant outcomes; only 35% of trials at low risk of bias.

    • Independent

      Naghsh 2023, inflammatory biomarkers after curcumin (umbrella meta-analysis)

      Umbrella meta-analysis of RCT meta-analyses · 10 meta-analyses, ~5,870 participants

      Authors declared no conflicts; pooled primary trials are of mixed, partly industry-funded provenance.

      Limits: High heterogeneity for CRP (I2=62%) and IL-6 (I2=76%); biomarkers are surrogates, not clinical outcomes.

    • Independent

      Nelson 2017, the essential medicinal chemistry of curcumin

      Critical medicinal-chemistry review · Narrative review (135 registered trials surveyed)

      Academic authors, no commercial stake; the strongest skeptical source.

      Limits: Narrative critique, not an outcome trial; addresses mechanism and reliability, not clinical efficacy directly.

    • Independent

      LiverTox (NIH/NIDDK), turmeric entry

      Authoritative clinical reference (case-series synthesis) · Several dozen documented cases

      NIH/NIDDK government resource, independent of industry.

      Limits: Idiosyncratic injury is rare and dose/formulation dependent; absolute risk per user is not quantified.

    • Independent

      Cochrane: reducing saturated fat for cardiovascular disease

      Systematic review & meta-analysis of RCTs · 15 RCTs, 56,675 participants

      Cochrane review; NIHR/academic support, no industry sponsor of the analysis.

      Limits: Cardiovascular events fell 17%; little effect on all-cause mortality on this timescale.

    • Mixed funding

      Engel & Tholstrup: butter vs olive oil on blood lipids

      Randomized double-blind crossover RCT · n=47

      University of Copenhagen; dossier notes partial dairy-sector support, unverified on the cited page, yet result was unfavorable to butter.

      Limits: Short (about 5-week periods); measures LDL, not clinical outcomes; butter also raised HDL.

    • Independent

      Harvey 2018: MCT and time to nutritional ketosis

      Randomized double-blind placebo-controlled trial · n=28 (23 completed)

      Human Potential Centre, AUT University; no-conflict declaration not visible on cited abstract.

      Limits: 20 days; faster time-to-ketosis not significant; ketone biomarker only, no lifespan endpoint.

    • Independent

      Khaw 2018: coconut oil vs butter vs olive oil

      Randomized controlled trial (3-arm) · n=94

      Investigator-led, Cambridge; NIHR/MRC support, no commercial product sponsor.

      Limits: 4 weeks; LDL endpoint, not clinical outcomes or lifespan.

    • Mixed funding

      Lu et al. 2020, Nature: reprogramming restores vision in mice

      Animal experimental (mouse), peer-reviewed · Multiple mouse cohorts (retinal ganglion cells)

      Academic/NIH plus foundation; Sinclair is a named inventor and Life Biosciences/Iduna equity holder (disclosed).

      Limits: Mouse-only; gene therapy, not a supplement; no lifespan endpoint.

    • Industry-funded

      Okabe et al. 2022, Frontiers in Nutrition: oral NMN safety and blood NAD+

      Randomized, double-blind, placebo-controlled trial · 30 healthy adults, 250 mg/day, 12 weeks

      Funded by Mitsubishi Corporation Life Sciences; several authors were employees who prepared the NMN/placebo.

      Limits: Endpoint was safety plus blood NAD+; no aging, healthspan, or lifespan outcome.

    • Industry-funded

      Sirtris/GSK resveratrol program history

      Corporate and clinical history (encyclopedia, cited reporting) · n/a

      Documents GSK's industry-funded SRT501 program and its 2010 termination.

      Limits: Secondary summary; underlying assay-artifact and shutdown facts well documented.

    • Industry-funded

      GSK halts SRT501 (analyst report, 2010)

      Industry/analyst report on a halted Phase 2a trial · Advanced multiple-myeloma patients; several developed renal failure

      Reports on GSK-funded clinical program.

      Limits: Single indication; documents failure, not aging efficacy.

    • Not applicable

      TAME metformin trial status (2024)

      News reporting on trial funding/status · Planned 3,000 adults aged 65 to 79, ~6-year trial

      Chronically underfunded; metformin is generic so no pharma sponsor.

      Limits: Not yet reported; no human aging outcome exists.

  • DetoxDetox products Unsupported
    • Funding unknown

      Klein & Kiat 2015, critical review of detox diets

      Critical narrative review (no human RCTs found) · Review of available clinical studies; authors note no human RCTs of commercial detox diets exist

      Funding/COI not shown on the abstract; peer-reviewed dietetics journal, University of Sydney / Macquarie University authors.

      Limits: Narrative not systematic; reflects scarcity of primary trials rather than resolving it.

    • Independent

      Kennedy et al. 2012, controlled ionic footbath test

      Proof-of-principle comparative trial (water, 24-hr urine, hair analysis) · n=6 healthy adults; 4 weekly 30-min sessions; measured to week 12

      Funded by the Holistic Health Research Foundation and Canadian CAM Research Fund; manufacturer donated the device but had 'no other involvement', null result despite a donated machine.

      Limits: Small (n=6) proof-of-principle design; adequate to show no signal, not a large RCT.

    • Independent

      Independent lab analysis of used foot pads (encyclopaedic summary)

      Tertiary summary of journalist-commissioned lab testing · na

      Underlying lab test was commissioned by journalists, not industry; used here only as a pointer to the primary finding.

      Limits: Tertiary source; cited as a signpost, not as primary evidence.

    • Independent

      Harvard Health, what is being cleansed in a cleanse

      Expert clinical commentary (medical school publication) · na

      Harvard Medical School publication; no industry funding.

      Limits: Commentary, not a primary study; synthesises existing evidence and documents harms.

    • Independent

      Cleveland Clinic, why foot detoxes do not work

      Expert clinical commentary · na

      Cleveland Clinic; no industry funding.

      Limits: Commentary, not a primary study.

  • HormonesDHEA Unsupported
    • Independent

      Nair 2006, NEJM, 2-year RCT in elderly with low DHEAS

      Randomized double-blind placebo-controlled trial (2 years) · n=144 (87 men, 57 women)

      NIH/NIA grants (P01 AG14283, M01 RR00585); no industry sponsor.

      Limits: Enrolled low-DHEAS people most likely to benefit, yet functional outcomes were null.

    • Funding unknown

      Corona 2013, JCEM, meta-analysis in elderly men

      Meta-analysis of RCTs · 25 trials, n=1,353, mean 36 weeks

      Funding/COI not disclosed; academic endocrinology authors.

      Limits: Small fat-mass effect vanished after adjusting for sex steroids; mostly short trials.

    • Independent

      Grimley Evans 2006, Cochrane, DHEA for cognition

      Cochrane systematic review of RCTs · Pooled small RCTs (~46-75 each)

      Cochrane Collaboration; no commercial sponsor.

      Limits: Few trials and limited data, but consistently null on cognition and wellbeing.

    • Funding unknown

      Zhu 2021, Steroids, estradiol dose-response in women

      Dose-response meta-analysis of RCTs · 21 arms, n=1,223 women

      Funding/COI not disclosed; supports only the biomarker point.

      Limits: Establishes estradiol rise, not any functional or anti-aging benefit.

  • EnvironmentalEMF Unsupported
    • Independent

      Karipidis 2024, WHO-commissioned systematic review

      Systematic review / meta-analysis of human observational studies · 63 studies, 22 countries, 119 exposure-outcome pairs

      Commissioned and part-funded by WHO; further support from NZ Ministry of Health, Istituto Superiore di Sanita, ARPANSA. No telecom-industry funding stated.

      Limits: Built on observational studies that can bound but never prove zero risk; a published ICBE-EMF critique disputes the conclusions.

    • Independent

      COSMOS prospective cohort (Feychting/Elwood 2024)

      Prospective cohort · 264,574 participants; median 7.1-year follow-up

      World Health Organization and UK Medical Research Council; authors declared no competing financial interests.

      Limits: Median follow-up still only ~7 years; relies on calibrated self-report plus operator data. Strength: exposure recorded before diagnosis, so immune to recall bias.

    • Independent

      UK Million Women Study (Schuz 2022)

      Prospective cohort · 776,156 women; 3,268 brain-tumour cases; ~14-year follow-up

      UK Medical Research Council and Cancer Research UK; authors declared no conflict of interest.

      Limits: Heavy attrition and early/coarse exposure assessment; very few heavy users, so weak for testing the heavy-use hypothesis.

    • Independent

      Danish nationwide cohort (Frei 2011)

      Nationwide register-based cohort · 358,403 subscribers; 3.8M person-years; 10,729 CNS-tumour cases

      Danish Strategic Research Council; Swiss National Science Foundation; no competing interests declared.

      Limits: Subscription used as exposure proxy misclassifies the heaviest (corporate) users, biasing toward the null.

    • Independent

      Little 2012, US glioma incidence vs phone uptake

      Ecological / incidence-trend · US SEER population, 1992-2008

      NIH Intramural Research Program; National Cancer Institute.

      Limits: Ecological design cannot detect a small individual-level effect; compatible with small INTERPHONE risks, incompatible with large Hardell risks.

    • Independent

      Zhang & Muscat 2025, SEER brain-tumour trends 2000-2021

      Ecological / incidence-trend · 20,325 adult and 2,372 paediatric CNS malignancies (SEER 22)

      Stated as not funded by any institution; authors declare no conflicts of interest.

      Limits: Ecological design; cannot establish individual causation, only test plausibility of large effects against real trends.

    • Independent

      NTP 2-year rat/mouse RF bioassay

      Animal carcinogenicity bioassay · ~3,000 rats and mice across exposure groups

      US Government (NIH/NIEHS). No industry funding.

      Limits: Whole-body exposure 9 hrs/day for 2 years at or above human SAR limits; clear effect only in male rats, ambiguous in females and mice; survival confound; NTP says do not extrapolate to humans.

    • Independent

      IARC 2011 Group 2B classification (Press Release N 208)

      Hazard classification / expert working-group statement · 31 scientists, 14 countries, reviewing hundreds of articles

      IARC (WHO agency); no commercial funding.

      Limits: A hazard label about strength of evidence, not a risk estimate; driven largely by one heavy-user finding (~40% raised glioma OR); the group did not quantify risk.

    • Independent

      Lu 2019, GrimAge predicts lifespan/healthspan

      Biomarker development + prospective mortality prediction (multi-cohort) · 7,375 arrays / 6,935 individuals (Framingham, WHI, Jackson Heart, InCHIANTI)

      NIH-funded academic work; UC/UCLA holds a patent with Lu and Horvath as inventors.

      Limits: Group-level prediction; not validated as a personal intervention-tracking tool.

    • Independent

      Belsky 2022, DunedinPACE pace-of-aging clock

      Biomarker development from longitudinal birth cohort · Dunedin 1972-73 cohort, ~1,000 members, 4 timepoints over 20 yrs

      NIA/MRC funding; Duke/Univ. of Otago hold a licensed patent, authors as inventors.

      Limits: High reliability (ICC ~0.96) but a research instrument; individual responsiveness to interventions not outcome-validated.

    • Independent

      Higgins-Chen 2022, reliability of clocks (Nature Aging)

      Methods/reliability re-analysis (technical replicates + longitudinal) · Six clocks on replicate and longitudinal methylation datasets

      NIA-funded; authors are patent inventors and disclose ties to TruDiagnostic/FOXO, yet conclusions are cautionary. (See source note: the abstract is verified; full disclosures are in the peer-reviewed paper.)

      Limits: Reliability shown mainly for first-generation clocks; PC versions fix most of it.

    • Independent

      Borrus 2024, false positives in intervention studies

      Re-analysis of six intervention datasets (preprint) · Six longitudinal intervention datasets

      NIA-funded (R01AG060110, R01AG065403); authors disclose consulting ties to TruDiagnostic, FOXO, Cambrian BioPharma, LongevityTech.fund.

      Limits: Preprint; re-analysis of existing data, not a new trial.

    • Independent

      Horvath 2013, foundational multi-tissue clock

      Methods/biomarker development (cross-sectional, multi-cohort) · ~7,844 non-cancer samples, 51 tissues/cell types

      UCLA academic; no consumer-test sponsorship in the 2013 paper.

      Limits: First-generation, trained on chronological age; later shown to have poor test-retest reliability.

    • Industry-funded

      Khavinson et al. 2003, Epitalon induces telomerase activity in human cells

      In vitro cell-culture experiment · Human telomerase-negative fetal fibroblasts; no organism-level n

      Authored by Epitalon's originators with commercial peptide-bioregulator interest; no explicit COI disclosure.

      Limits: In vitro only; originator-authored; cited link is paywalled (verified via Europe PMC, PMID 12937682).

    • Independent

      Al-dulaimi et al. 2025, Epitalon increases telomere length in human cell lines

      In vitro independent replication · Multiple human cell lines (21NT, BT474, IBR.3, HMEC; U2OS, PC3-hTERT controls)

      Self-funded PhD students plus departmental support; authors declared no competing interests; not affiliated with Khavinson.

      Limits: 2D culture only; qPCR averages telomere length; no in vivo or human validation; confirms cancer-cell lengthening via ALT.

    • Industry-funded

      Khavinson & Morozov 2003, Peptides of pineal gland and thymus prolong human life

      Long-term clinical follow-up (PubMed-indexed RCT) · 266 elderly subjects, 6 to 8-year follow-up

      Originator-authored; no COI disclosure; low-impact journal.

      Limits: Sole basis for the human claim; abstract describes no blinding or placebo; implausibly large dose-escalating effects; never independently replicated.

    • Funding unknown

      Superpower regulatory and evidence status overview 2026

      Secondary status review · n/a

      Commercial health-content site; cited only for regulatory and replication status, not efficacy; states it does not sell or compound Epitalon.

      Limits: Secondary source; not efficacy evidence.

    • Industry-funded

      Wei et al. 2017, Sci Transl Med (pivotal RCT)

      Randomized controlled crossover trial · 100 randomized; 71 completed 3 cycles

      USC chair fund (Longo); FMD supplied by L-Nutra; Longo and a co-author held equity and recused from data collection/analysis; equity pledged to Create Cures.

      Limits: Modest size, short term; key glucose/lipid/CRP cuts only in pooled/post hoc, not primary comparison.

    • Industry-funded

      Brandhorst et al. 2024, Nat Commun ('biological age')

      Secondary/exploratory analysis of two trials · Pooled 100 + 44 participants

      Longo (senior author) founded L-Nutra; FMD is L-Nutra's ProLon; formulations belong to USC and L-Nutra. Full COI paragraph not extracted from page.

      Limits: Surrogate blood-marker clock, not epigenetic; secondary endpoint; no active-CR comparator; no hard outcomes.

    • Industry-funded

      USC press release 2024

      University press release · n/a

      Issued by Longo's institution; USC discloses a financial interest in L-Nutra.

      Limits: Source of 'rejuvenation' and 'biologically younger' framing; not independent evidence.

    • Independent

      Robbins, STAT News 2017

      Independent investigative journalism · n/a

      No industry funding of the reporting.

      Limits: Documents the commercial conflict; quotes independent scientists noting weight loss may explain benefits and head-to-head human comparisons are lacking.

    • Independent

      Cochrane review, water fluoridation for caries (2024)

      Systematic review / meta-analysis · 157 studies screened; key contemporary dmft estimate from 2 studies / 2,908 children

      Cochrane Oral Health; no commercial sponsorship.

      Limits: Contemporary estimates from only 2-4 modern studies, low/very-low certainty (confounding, no blinding); CI crosses null.

    • Independent

      NTP monograph, fluoride and neurodevelopment (2024)

      Federal systematic review / monograph · >70 studies across 12 countries; 22 rated high-quality

      US government NIEHS/NTP; no industry funding.

      Limits: Observational, mostly high-fluoride regions abroad; residual confounding; confidence capped at moderate (>1.5 mg/L) and insufficient at 0.7 mg/L.

    • Independent

      Cochrane plain-language summary (2024)

      Systematic review (plain-language summary) · Same evidence base as the full review

      Cochrane Oral Health.

      Limits: Summary only; reiterates that present-day benefit is small now toothpaste is widespread.

    • Not applicable

      Food & Water Watch v. EPA, district ruling (2024)

      Federal district court ruling (legal, not scientific) · n/a

      Court proceeding; not a funded study.

      Limits: Used lower TSCA 'preponderance' bar; explicitly not a finding of injury; ordered review only.

    • Not applicable

      Ninth Circuit vacatur (May 2026)

      Federal appellate ruling (procedural) · n/a

      Court proceeding; not a funded study.

      Limits: Vacated on procedure; explicitly did not decide whether 0.7 mg/L poses unreasonable risk.

    • Independent

      ACMG practice guideline (Hickey 2013)

      Clinical practice guideline / evidence review · Synthesis of multiple meta-analyses

      Professional-society guideline (ACMG); no commercial sponsor.

      Limits: Guideline, not new primary data; focused on thrombophilia/CHD endpoints.

    • Independent

      Homocysteine in the cardiovascular setting (2025 review)

      Narrative review of large RCTs · HOPE-2 ~5,500; NORVIT ~3,700; VITATOPS ~8,000

      Academic review of publicly funded RCTs; no industry sponsor identified.

      Limits: Narrative, not a fresh meta-analysis; HOPE-2 did show a stroke-incidence drop though the primary CVD endpoint was null.

    • Funding unknown

      Methylfolate/folinic acid RCT in healthy adults (2023)

      Randomized controlled trial (biomarker endpoint) · 272 adults, 3 months

      Funding not stated in abstract; treat as unverified.

      Limits: Biomarker-only (homocysteine); no clinical or healthspan outcomes; short duration.

    • Independent

      MTHFR genetic-counseling review (Levin & Varga 2016)

      Evidence-based clinical review · Literature synthesis

      Peer-reviewed journal; no industry sponsor identified.

      Limits: Publisher full text paywalled; thrust confirmed via PubMed, exact quotes not verbatim-verified.

    • Independent

      Bernardes de Jesus & Blasco, AAV9-TERT in mice (EMBO Mol Med, 2012)

      In vivo animal intervention (vector-group randomized) · 1-yr group n=52 (TERT 21/eGFP 14/dead-TERT 17); 2-yr group n=37 (TERT 23/eGFP 14)

      Spanish National Cancer Research Centre (CNIO); authors declared no conflict

      Limits: Mice only; lifespan DELAY not reversal; no-cancer result partly mouse-specific (post-adult dosing, non-integrating diluted vector).

    • Independent

      Zhang et al., telomere length and cancer, Mendelian randomization (Hum Mol Genet, 2015)

      Human genetic epidemiology (Mendelian randomization) · Multi-consortium GWAS, tens of thousands across cancers

      NCI/NIH (GAME-ON), Wellcome Trust, Australian NHMRC

      Limits: Genetic-proxy, not interventional; informs causal cancer risk, not telomerase-therapy safety directly.

    • Not applicable

      Regalado, "Buyer beware of this $1M gene therapy for aging" (MIT Technology Review, 2019)

      Investigative journalism / expert reporting · n/a (claimed 2-3 prospective patients; CRO said none enrolled)

      Independent journalism; subject venture was pay-to-participate ($1M/patient)

      Limits: Reporting, not primary trial data; enrollment claims conflicted across sources.

    • Independent

      Gorski, $1M phase 1 anti-aging gene therapy critique (Science-Based Medicine, 2019)

      Physician-scientist critical analysis · n/a

      Independent academic medical commentary

      Limits: Commentary, not new data; argues phase 1 tests safety only, so charging $1M is unjustified.

    • Not applicable

      $1M anti-aging gene therapy raises ethical concerns (Genetic Literacy Project, 2019)

      Science journalism (republished aggregation) · n/a

      Republished excerpt of Emily Mullin's OneZero piece; corroborating, not original

      Limits: Aggregation, not independent reporting; confirms offshore/pay-to-play but not the cancer caveat.

    • Industry-funded

      Pickart & co. 2015 review (source of the 4,000-gene "reset")

      Narrative review (discoverer-authored) · N/A

      Skin Biology affiliation; sells GHK.

      Limits: The 4,000-gene figure is in-silico Connectivity Map matching, not validated in human tissue.

    • Industry-funded

      Pickart & Margolina 2018 review

      Narrative review (discoverer-authored) · N/A

      Skin Biology; declares 'no conflict' despite commercializing GHK.

      Limits: Reports 31.2% of genes affected via CMap (in-silico); human data limited to small topical creams.

    • Independent

      Dou et al. 2020 (independent UW review)

      Narrative review (independent of discoverer) · N/A

      Univ. of Washington; NIA-funded, no Skin Biology tie.

      Limits: Frames anti-aging as hypothesis; confirms human evidence limited to topical skin.

    • Funding unknown

      Leyden et al. 2002 facial-cream study

      Cosmetic study, conference abstract (not peer-reviewed) · n=71, 12 wk, topical

      Authors reportedly industry-affiliated; not documented in the cited source.

      Limits: Abstract only, no full RCT; favourable result.

    • Industry-funded

      Yuvan Research 2023 collagen-density study

      Company-sponsored study, press release (not peer-reviewed) · n=21, 3 mo, topical

      Yuvan Research sells the tested gel.

      Limits: Surrogate ultrasound endpoint; small n; unpublished.

    • Independent

      Kumar 2021 open-label pilot (Sekhar/Baylor)

      Open-label pilot, no placebo, single-group · 8 older + 8 young adults, 24 wk + 12 wk washout

      Funded by a philanthropic gift from the McNair Medical Institute. (No NIH/NIA grant is stated in this paper.) Lead author holds a GlyNAC patent.

      Limits: Tiny, uncontrolled; benefits regressed after withdrawal; patent conflict not disclosed.

    • Independent

      Kumar 2023 randomized trial (Sekhar/Baylor), NCT01870193

      Randomized double-blind placebo-controlled, 16 wk · 24 older adults (12 GlyNAC / 12 placebo) + 12 young

      NIH/NIA R01AG041782 + McNair gift (verified). COI stated 'None declared' despite Sekhar's GlyNAC patent: a material non-disclosure.

      Limits: n=24; surrogate endpoints only; 'aging hallmarks' framing relabels biomarker shifts; single group.

    • Industry-funded

      Lizzo 2022 (Nestle), Frontiers in Aging

      Randomized double-blind placebo-controlled dose-ranging, 14 days · 117 older + 20 young adults

      Funded by Nestle Health Science (markets glycine+NAC); ~6 of 9 authors Nestle employees; patents/licenses disclosed.

      Limits: Null overall for glutathione/MDA; benefit only post-hoc subgroup; short 14-day duration may partly explain.

    • Independent

      Kumar 2022 mouse lifespan study (Sekhar/Baylor)

      Preclinical animal (mouse) lifespan study · Mice (C57BL/6J)

      Stated as internal Baylor College of Medicine funding (not NIH/NIA or McNair). Same investigator patent conflict applies.

      Limits: Mouse-only +~24% lifespan; does not establish any human lifespan or healthspan effect.

    • Not applicable

      US Patent 9,084,760 B2 (Sekhar / Baylor)

      Patent / intellectual-property record · Not applicable

      Granted 2015; claims combined glycine + N-acetylcysteine; Baylor licensed GlyNAC IP to Nestle Health Science.

      Limits: Establishes the investigator's commercial interest; not disclosed in the pivotal 2023 trial.

    • Industry-funded

      Ghaly & Teplitz 2004, cortisol & sleep

      Uncontrolled pilot, subjective endpoints · n=12, ~8 weeks grounded during sleep

      Tied to the Ober/Earthing Institute promotion network; full-text COI not verifiable at source.

      Limits: No control group or blinding; sleep, pain, stress self-reported. Most-cited foundational study.

    • Industry-funded

      Chevalier 2013, blood viscosity

      Small uncontrolled clinical study · n=10 healthy adults, 2 h grounding

      PubMed lists 'Research Support, Non-U.S. Gov't'; same EarthFx-linked authors (Chevalier, Sinatra, Oschman).

      Limits: Surrogate marker (RBC zeta potential) only; no clinical outcome; no control group; n=10.

    • Industry-funded

      Oschman 2015, inflammation review

      Narrative review (proponent-authored) · N/A; cites pilots incl. an 8-subject DOMS study

      Chevalier & Oschman are EarthFx contractors AND shareholders; Brown a contractor only. COI real for all three.

      Limits: Asserts electron/antioxidant mechanism; authors concede pilots had 'relatively few subjects.'

    • Funding unknown

      Park et al. 2025, sleep RCT

      Randomised double-blind placebo-controlled pilot · n=60, 31-day intervention, sham-mat control

      Kyung Hee University; no external funding disclosed in abstract. More independent than EarthFx literature.

      Limits: Subjective sleep/stress endpoints; pilot scale; makes NO anti-inflammatory or disease-modifying claim.

    • Independent

      Africa Check appraisal

      Independent fact-check · N/A

      Non-profit fact-checking organisation; consulted physicist Chad Orzel.

      Limits: Secondary appraisal; concludes evidence insufficient, replication mixed (2010 positive vs 2015 null).

    • Industry-funded

      Hachmo et al. 2020, Aging (the pivotal trial)

      Single-arm, uncontrolled, unblinded, non-randomized prospective trial; surrogate blood-cell biomarkers only · 35 enrolled; 25 analyzed for telomeres, 20 for senescent cells; healthy adults aged 64+

      Private Sagol-network grant. Per the paper's own disclosure, co-authors are employees of AVIV Scientific LTD and 'ES' (Efrati) is a shareholder, the company selling the anti-aging HBOT program the study markets. Direct seller conflict.

      Limits: No control or sham group, no randomization, no blinding; small, high-attrition sample (~57% of enrollees analyzed for senescence); outcomes are surrogate markers in isolated blood cells only; no clinical, functional, or lifespan endpoint.

    • Industry-funded

      Tessema et al. 2022, Frontiers in Aging (systematic review)

      Systematic review of hyperoxia and aging biomarkers (17 studies, 9 human) · 17 studies pooled; 9 human trials plus cell/animal; pooled n not reported

      Article processing charge funded by InterHypox e.V.; supported by CellAir Construction GmbH; one author (Egorov, 'EE') is co-owner of CellAir, a hyperoxia-equipment company. Seller-adjacent conflict.

      Limits: Most included studies rated high/unclear risk of bias; concludes there is no direct research showing short-term hyperoxia increases human life expectancy; documents harms (visual acuity loss, barotrauma, mtDNA damage, cataract risk).

    • Independent

      Reason, Fight Aging! 2020 (independent critique)

      Independent expert commentary · N/A

      Independent longevity-research commentator; no commercial stake in HBOT.

      Limits: Commentary, not new data; argues immune-cell telomere length is a poor aging readout and that apparent changes likely reflect immune-cell population shifts rather than systemic rejuvenation.

    • Independent

      Schneider / Brown, For Better Science 2020 (statistical critique)

      Independent investigative and statistical critique · N/A

      Independent science-integrity journalism; no commercial interest in HBOT.

      Limits: Critique, not new data; flags non-independent subset comparisons ('Table 1 is absurd'), unreconciled participant counts, and inconsistent exclusion of low-quality samples (4 vs 10) from identical preparations.

    • Not applicable

      Gulf News 2021 (money-trail report)

      News / business report (commercial context) · N/A

      Mainstream news outlet; cited to document the commercial product, not as scientific evidence.

      Limits: Documents the Dubai clinic launch of a 60-session anti-aging HBOT program under Efrati's scientific leadership; reports treatment cost 'typically within $80,000'; does not establish a Florida site or any clinical outcome.

    • Independent

      Liu 2007, systematic review of GH in healthy elderly

      Systematic review/meta-analysis (18 RCT populations) · ~220 GH-treated pooled

      NIH (NIA) / AHRQ; no industry conflict.

      Limits: Trials short (months), powered for body composition, not clinical outcomes.

    • Independent

      Blackman 2002, GH +/- sex steroids RCT

      Randomized double-blind placebo-controlled, 26 weeks · 131 adults aged 65-88

      NIH/NIA intramural and academic.

      Limits: Short; strength gains absent in women; high adverse-event rates.

    • Independent

      Perls 2005, clinical-legal commentary

      Expert clinical-legal analysis · N/A

      NIH/NIA-supported academics; lead author a known industry critic.

      Limits: Commentary, not a trial; legal facts cite the statute.

    • Independent

      Laron-syndrome cohorts (low IGF-1)

      Longitudinal observational cohorts · 230 patients vs relatives

      Academic/foundation-funded; no GH-industry sponsor.

      Limits: Small, specific populations; protected from disease but did not outlive relatives on average.

    • Funding unknown

      Bauer 2013 PROT-AGE position paper

      Expert consensus guideline · N/A (expert review)

      EUGMS-appointed group; individual author industry ties common in this field but not extracted.

      Limits: Extrapolates from mechanistic and short-term functional data; no long-outcome RCTs at these intakes.

    • Independent

      Volkert 2019 ESPEN geriatric guideline

      Clinical practice guideline · N/A (guideline)

      ESPEN professional-society process.

      Limits: At-least-1.0 g/kg/day figure lives in full-text recommendations, not the abstract; consensus, not trial outcome.

    • Independent

      Levine 2014 (NHANES III + mice)

      Observational cohort + animal · 6,381 adults; plus mouse cohorts

      Academic/USC-Longo, NIH/foundation; Longo has commercial ties to ProLon/L-Nutra aligned with a restriction message.

      Limits: Single 24-hour recall; animal protein confounded by red/processed meat and lifestyle; also showed ~5x diabetes-mortality rise at all ages.

    • Industry-funded

      Devries 2018 kidney meta-analysis

      Meta-analysis of RCTs · 28 RCTs, 1,358 participants

      Senior author Phillips disclosed dairy- and beef-industry funding; favorable conclusion warrants scrutiny though consistent with literature.

      Limits: Short trials; applies only to normal kidney function, not chronic kidney disease.

    • Independent

      Science Media Centre expert reaction

      Independent expert commentary · N/A

      Collates independent academic commentary.

      Limits: Commentary, not new data; critiques the strength of the Levine harm signal.

    • Independent

      St Hilaire et al. 2012, human phase response curve to a 1 h bright-light pulse

      Randomized laboratory phase-response (constant routine) study · 34 participants (18 bright light, 16 dim control)

      NIH/NIMH, NHLBI, NASA/NSBRI, Wellcome Trust; authors declared no conflicts related to the work.

      Limits: Single 1 h pulse at ~8,300 lux under controlled dim-light lab conditions; small n; not a real-world outdoor exposure.

    • Independent

      Khalsa et al. 2003, phase response curve to single bright light pulses

      Laboratory phase-response (constant routine) study · 21 subjects

      NIH grants (incl. NIMH R01-MH45130, NHLBI); academic/public funding, no product sponsor.

      Limits: High-intensity (~10,000 lux) pulses of ~6.7 h, far longer/brighter than a brief outdoor glance; intensive small cohort.

    • Independent

      Czeisler 1995, the effect of light on the human circadian pacemaker (review)

      Foundational human circadian physiology review · na

      NIH grants (NIA, NIMH); public/academic funding, no product sponsor.

      Limits: Narrative/review synthesis establishing light as primary zeitgeber; not a single dose-response trial.

    • Independent

      Perera, Eisen, Bhatt, Bhatnagar, de Souza, Thabane, Samaan 2016, light therapy for non-seasonal depression (meta-analysis)

      Systematic review and meta-analysis of RCTs · 20 RCTs, 881 participants

      Senior author supported by Canadian Institutes of Health Research and a Brain & Behavior Research Foundation grant; declared no conflicts.

      Limits: Substantial heterogeneity (I-squared=60%); high risk of bias (only ~5/21 low risk); blinding of bright light intrinsically hard; population is clinically depressed, not healthy.

    • Industry-funded

      Siraji et al. 2022, daytime electric light, alertness and cognition (systematic review)

      Systematic review · 59 studies; mean per-study n approximately 35

      Supported by a research grant from ITRAMAS Corporation, a lighting manufacturer; authors declared no competing commercial relationship.

      Limits: Heterogeneous designs; subjective alertness improved in ~65% but objective in only ~31% of studies; industry-funding flag noted; tempers rather than confirms the alertness claim.

    • Funding unknown

      He, Ru, Li, Li, Zhou 2023, morning bright light improves nocturnal sleep and next-morning alertness in college students

      Controlled crossover field intervention · 12 participants

      No funding or conflict-of-interest statement visible in the abstract record.

      Limits: Very small n; 1.5 h dose (about 90x the prescribed glance); objective sleep measures significant but several self-rated measures were not.

    • Independent

      Nussbaumer-Streit et al. 2019, light therapy for preventing seasonal affective disorder (Cochrane review)

      Cochrane systematic review · One eligible prevention RCT (46 participants)

      Cochrane methodology; no product sponsor for the review itself.

      Limits: Only one small prevention trial; evidence rated very low; no firm conclusion on prevention possible (treatment of active SAD is separately well established).

    • Not applicable

      Huberman Lab sponsors page and ROKA partnership (primary commercial disclosure)

      Primary source (claimant commercial disclosures) · na

      Claimant's own commercial relationships (AG1, Eight Sleep, Helix, WHOOP, LMNT, Joovv, ROKA); relevant to conflict-of-interest transparency, not evidence for the claim.

      Limits: Not scientific evidence; documents the money trail only. No equity/ownership stake verifiable from the sources reviewed.

    • Independent

      TREAT RCT (Lowe 2020)

      Randomized controlled trial · n=116, 12 weeks

      NIH/NIDDK. Authors held Keyto/Virta ties that would favour fasting, yet the result was null, which strengthens it.

      Limits: Short; modest size; ~65% of the small weight loss was lean mass (P=0.005).

    • Independent

      CR with/without TRE (Liu 2022, NEJM)

      Randomized controlled trial · n=139, 12 months

      Chinese public research grants; no industry weight-loss sponsor.

      Limits: Single-centre; only early (8am-4pm) window; primary URL paywalled, numbers confirmed via PubMed.

    • Independent

      IF vs continuous CR meta-analysis (He 2022)

      Systematic review and meta-analysis · 11 RCTs, n=705

      Chinese public foundations; no diet-industry funding.

      Limits: Heterogeneous protocols; short trials; tiny weight advantage of uncertain clinical value.

    • Independent

      Early TRE feeding trial (Sutton 2018)

      Randomized crossover feeding trial · n=8 men, 5 weeks/arm

      NIH grants; academic, no diet-product sponsor.

      Limits: Only 8 men; early window only; not replicated at scale.

    • Independent

      Autophagy flux analysis (Bensalem 2025)

      Exploratory analysis nested in an RCT · n=121, 6 months

      Australia NHMRC and Diabetes Australia; public/academic.

      Limits: Surrogate blood-cell marker; within-group null; exploratory; no outcome link.

    • Funding unknown

      NHANES 8-hour window and CV death (Zhong 2024, AHA abstract)

      Observational cohort (conference abstract, not peer-reviewed) · ~20,000 adults, ~8-year follow-up

      Conference abstract; funding not disclosed.

      Limits: Diet from two days of recall; reverse causation and confounding likely; not peer-reviewed.

    • Independent

      Ali et al. 2009 - Myers' cocktail RCT (fibromyalgia)

      Double-blind, placebo-controlled pilot RCT · 34 randomized (16 infusion, 18 lactated-Ringer's placebo); 31 completed; weekly x8 weeks + follow-up to 12

      US NIH/NCCAM grants R21AT000942 and F32AT00667; authors declared no competing interests.

      Limits: Tiny pilot; single condition; primary outcome null (between-group p=0.60 / 0.39); both arms improved similarly, consistent with placebo response.

    • Industry-funded

      Suh et al. 2012 - IV vitamin C and fatigue

      Double-blind, placebo-controlled RCT · 141 healthy office workers (70 vitamin C, 71 saline); single 10 g infusion

      Korean Association for Vitamin Research; two authors held leadership/membership roles in the funding body (sponsor stated to have no role).

      Limits: Subjective 0-10 endpoint; effect confined to LOW-baseline-vitamin-C subgroup (p=0.004) with none in adequate-baseline (p=0.206); single infusion, short follow-up; unreplicated; no oral comparator, so cannot test IV-vs-diet.

    • Independent

      Drug & Therapeutics Bulletin 2023 - evidence review

      Independent evidence review / editorial bulletin · Narrative review (no pooled n)

      Drug & Therapeutics Bulletin (BMJ), editorially independent, advertising-free; none declared.

      Limits: Narrative, not a meta-analysis; reflects scarcity of primary trials rather than a pooled effect estimate.

    • Independent

      Alangari 2025 (Cureus) - narrative review of IV vitamin therapy

      Narrative review · Narrative review (no participants)

      Author declares no financial support, financial relationships or conflicts of interest.

      Limits: Narrative, not a systematic review/meta-analysis; catalogues risks and the unstandardized formulations but pools no primary data.

    • Independent

      CSPI/A4PC 2025 (JAMA Internal Medicine) - state oversight of IV hydration spas

      Cross-sectional policy/regulatory analysis (research letter) · 50 US states + DC (policy/practice survey)

      Center for Science in the Public Interest and Alliance for Pharmacy Compounding; published as a JAMA Internal Medicine research letter; no stake in selling drips.

      Limits: Documents the regulatory gap, not clinical efficacy; policy snapshot as of mid-2024.

    • Independent

      Cochrane review, keto for drug-resistant epilepsy

      Systematic review/meta-analysis of 13 RCTs · 932 participants (711 children, 221 adults)

      Academic Cochrane synthesis; no dominant industry sponsor among included trials.

      Limits: All trials unblinded, high risk of bias; certainty low to very low; adults showed no seizure freedom.

    • Independent

      Newman/Verdin, keto in aging mice

      Animal (mouse) longevity/healthspan · Lifespan cohort n=241 male mice

      NIH grants plus Glenn/AFAR/Hillblom foundations; no commercial COI.

      Limits: Cyclic diet to avoid obesity; reduced midlife mortality but NO maximum-lifespan gain; male mice only.

    • Independent

      Roberts et al., keto extends longevity in mice

      Animal (mouse) longevity/healthspan · Longevity cohort n=43-44 male mice

      NIH (NIDDK/NIA) grants; no commercial COI captured.

      Limits: Increased median lifespan only; exact effect size not in abstract; male mice only.

    • Industry-funded

      Virta Health continuous-care trial, type 2 diabetes

      Non-randomized prospective clinical trial · ~262 intervention vs ~87 usual care

      Sponsored by Virta Health, which sells a keto diabetes program; most authors employed/with stock.

      Limits: Non-randomized, self-selected; shows metabolic benefit, not longevity; direct commercial COI.

    • Industry-funded

      KETO-CTA, LDL and plaque in hyper-responders

      Prospective observational cohort, CT angiography · 100 lean mass hyper-responders, 1-year follow-up

      Crowd-funded via Citizen Science Foundation, led by low-carb advocate Dave Feldman.

      Limits: No control arm, only 1 year; does not overturn the ApoB-cardiovascular evidence base.

    • Funding unknown

      Mah & Pitre 2021, oral magnesium for insomnia (systematic review)

      Systematic review & meta-analysis of RCTs · 3 RCTs, 151 older adults with insomnia

      No funding stated; COI 'none declared.' Academic clinicians, no obvious industry sponsor.

      Limits: All trials moderate-to-high risk of bias; certainty low to very low; authors call the literature 'substandard.'

    • Independent

      Fang 2016, dietary magnesium and mortality (dose-response meta-analysis)

      Dose-response meta-analysis of prospective cohorts · 40 publications, >1 million participants, 4-30 yr follow-up

      Chinese government foundations (National Natural Science Foundation; Zhejiang Provincial). Not industry.

      Limits: Dietary intake (food questionnaires), not supplements; total CVD and CHD non-significant; residual confounding precludes causal inference.

    • Funding unknown

      von Luckner & Riederer 2018, magnesium for migraine prophylaxis (systematic review)

      Systematic review of RCTs · 5 trials (from 204 screened)

      Academic neurology review; no industry sponsor identified.

      Limits: Mixed results; only Grade C ('possibly effective'); high dose (~600 mg/day dicitrate). A narrow, separate use from sleep/longevity.

    • Industry-funded

      Lopresti & Smith 2026, magnesium L-threonate (Magtein) on cognition and sleep (RCT)

      Randomized, double-blind, placebo-controlled trial · 100 adults, 6 weeks, 2 g/day Magtein (145 mg elemental Mg)

      Funded by Threotech Inc., the Magtein manufacturer, which supplied the product and helped design the study; lead author directs the contract research organisation. Clear conflict of interest.

      Limits: Short, small, surrogate-heavy; improved subjective sleep (p=0.043) but NO objective (Oura) sleep gain; markets a '~7.5-year cognitive age' figure.

    • Independent

      Papagiannidou 2026, hypomagnesemia clinical & nutritional update (review)

      Narrative/clinical review (NHANES-informed) · General-population prevalence estimates

      Academic review drawing on government survey (NHANES); no industry funding.

      Limits: Clinically measured hypomagnesemia ~1.5-15%; up to ~48% of US adults below estimated average requirement; serum poorly reflects total-body stores, so true deficit prevalence is uncertain.

    • Industry-funded

      Cleveland Clinic FM cohort (JAMA Netw Open 2019)

      Retrospective cohort (not randomized) · 398 matched pairs at 6mo; 220 at 12mo

      Center evaluated its own model; one author took fees from Cleveland HeartLab Inc., another was a paid Institute for Functional Medicine consultant/teacher.

      Limits: Sub-threshold 6mo gain; non-significant at 12mo (P=.41); ~14% retention; conceded nonresponse bias.

    • Independent

      Gorski, Science-Based Medicine (2018)

      Expert critique (professor of surgery & oncology) · N/A

      Independent science-advocacy publication; no commercial stake.

      Limits: Commentary, not primary data; argues FM tests are not evidence-based.

    • Independent

      Jarry, McGill OSS (2020)

      Expert critique (science communicator, M.Sc.) · N/A

      University science-outreach office; no commercial stake.

      Limits: Commentary; argues 'root cause' framing is 'an alluring falsehood.'

    • Not applicable

      Function Health (company/financial record)

      Company and financial record · ~160 tests; ~$2.5B valuation (Nov 2025)

      For-profit firm Hyman co-founded; documents the money trail, not clinical efficacy.

      Limits: Not clinical evidence; establishes a testing-and-supplement incentive.

    • Mixed funding

      PREDIMED (republished RCT, primary prevention)

      Randomized controlled trial · 7,447 high-risk adults

      Mainly Spanish public funding; olive oil and nuts donated by food companies, reportedly no sponsor role in design.

      Limits: Powered for events, not mortality; high-risk Spanish population; low-fat control was poorly adhered.

    • Independent

      Harvard Nutrition Source: retraction/republication explainer

      Authoritative summary · Same trial (7,447)

      Academic public-health institution; no commercial funding.

      Limits: Secondary summary, not primary data.

    • Independent

      Sofi 2008 meta-analysis (cohorts)

      Meta-analysis of prospective cohorts · ~1.57 million across 12 cohorts

      Authors declared 'Funding: None' and no competing interests.

      Limits: Observational; residual confounding and healthy-user bias.

    • Funding unknown

      Lyon Diet Heart Study (secondary prevention)

      Randomized controlled trial · 605 post-MI patients

      Academic/public-sector trial per dossier; funding not shown on source page.

      Limits: Small; older trial; funding not directly verifiable from the cited page.

  • DrugsMetformin Unproven
    • Funding unknown

      Bannister 2014, UK CPRD observational cohort

      Retrospective observational cohort · ~78,241 metformin + ~12,222 sulphonylurea vs ~90,463 non-diabetic controls; 503,384 person-years

      Often cited as AstraZeneca/Bristol-Myers Squibb funded; PubMed lists only 'Research Support, Non-U.S. Gov't' and the publisher disclosure is paywalled. Industry funding asserted on secondary sources only, not independently verified.

      Limits: Observational; healthy-user, prevalent-user and indication bias; comparison structure cannot establish lifespan extension. Authors themselves caution against an anti-aging reading.

    • Independent

      Strong 2016, NIA Interventions Testing Program (mice)

      Randomized controlled animal lifespan study (3 sites) · Genetically heterogeneous mice; multi-cohort across Jackson Lab, U Michigan, UT Health San Antonio

      US National Institute on Aging (AG013319, AG022303/07/08, AG024824). No industry funding.

      Limits: Animal model; dosing/timing differs from human protocols. Note: combo benefit vs rapamycin alone was not statistically significant (P=0.12), so 'attributable to rapamycin' is suggestive, not proven.

    • Mixed funding

      Konopka 2019, exercise-adaptation RCT

      Double-blind randomized controlled trial · 53 older adults (~62 yrs): placebo n=26, metformin n=27; 12 weeks

      Dairy Management Inc. and Dexcom Inc.; authors declared no conflicts. Not funded by metformin makers and funders had no stake in a negative result, strengthens credibility.

      Limits: Small (n=53); the VO2max attenuation did not reach significance (p=0.08). Suggestive signal of harm, not definitive.

    • Funding unknown

      Mohammed 2021, critical review

      Critical narrative review · N/A (review)

      Funding not stated in accessed text; authors academic pharmacologists (Weill Cornell-Qatar / U Calgary).

      Limits: Narrative synthesis, not primary data. Concludes the human lifespan-extension evidence in disease-free people 'remains controversial.'

    • Mixed funding

      AFAR, TAME trial (status)

      Planned RCT (not yet enrolling) · Planned ~3,000 adults aged 65-79, ~6 years, 14 sites; not yet enrolled

      Coordinated by AFAR; reliant on philanthropy and NIH negotiations. No results to bias; listed for status only.

      Limits: Not launched; no data. Documents why high-quality human evidence is absent.

    • Independent

      de Jager 2010 (HOME trial), B12 outcome

      Randomized placebo-controlled trial · 390 patients; ~4.3 years

      Investigator-led HOME trial. Demonstrates harm, so no favourable-sponsor concern.

      Limits: Conducted in diabetics; absolute risk-benefit in healthy non-diabetics is inferred. B12 fell ~19%; absolute deficiency risk rose ~7 percentage points.

    • Funding unknown

      Infante 2021, B12 safety review

      Narrative/mini-systematic review · N/A (review)

      Authors Infante M, Leoni M, Caprio M, Fabbri A; declared no competing interests; no funding stated.

      Limits: Review, not primary data; corroborates GI intolerance and long-term B12 depletion but does not itself report the 19%/7pp figures (those are de Jager's).

    • Independent

      Rodriguez 2016, Radiology: single-dose fMRI RCT

      Double-blind placebo-controlled crossover pilot · 26 healthy adults

      NIH grants (NIA, NCATS); no commercial sponsor.

      Limits: Tiny n, single acute dose, imaging/short-task surrogate; no durable or 'energy' endpoint.

    • Funding unknown

      Yang/Liu 2015, Prog Neurobiol: mechanism review

      Narrative review (mitochondria, cells, rodents) · No primary human cohort

      No funding/COI captured.

      Limits: Predominantly preclinical; human Alzheimer's data flagged as methodologically weak.

    • Independent

      Ramsay 2007, Br J Pharmacol: MAO-A inhibition

      In vitro enzyme kinetics · Recombinant human MAO enzymes

      Authors declared no conflict of interest.

      Limits: Biochemical basis for serotonin toxicity, not a clinical outcome study.

    • Mixed funding

      Xue 2021, Cells: anti-aging review

      Narrative review (anti-aging/mitochondrial) · No human longevity cohort

      NIH R01HL126784; author K.C. founded skincare firm Mblue Labs (conflict for skin/aging claims).

      Limits: Anti-aging evidence cell/animal; Alzheimer's phase 3 (LMTM) inconclusive; no human lifespan proof.

    • Independent

      Xiong 2017, Sci Rep: skin anti-aging

      In vitro fibroblasts + 3D skin models · Cultured cells and reconstructed skin (no human in vivo arm)

      Maryland Innovative Initiative; this paper declares no competing interests.

      Limits: Surrogate, cosmetic-level; no living-volunteer or systemic longevity data.

    • Funding unknown

      Leslie 2022, plastic in human blood

      Cross-sectional detection (proof of presence) · 22 healthy adult donors

      Indexed as non-commercial academic; specific funder not stated in abstract.

      Limits: Detection only; makes no health-harm claim; funder not visible.

    • Funding unknown

      Ragusa 2021, "Plasticenta"

      Cross-sectional detection (proof of presence) · 6 human placentas

      Indexed 'Research Support, Non-U.S. Gov't'; no commercial conflict identified.

      Limits: Very small n; detection only; one fragment confirmed polypropylene, rest pigment-identified.

    • Independent

      Marfella 2024, microplastics in atheroma

      Prospective observational cohort (no causation) · 257 carotid-endarterectomy patients

      Academic (Univ. of Campania Luigi Vanvitelli); reported not industry-funded.

      Limits: Association only; possible sample contamination; residual confounding; full text paywalled, figures from PubMed/secondary reporting.

    • Independent

      WHO, microplastics risk assessment

      Authoritative risk-assessment review · Evidence synthesis

      WHO; no commercial interest.

      Limits: Concludes evidence base is limited and insufficient for firm toxicity conclusions.

    • Mixed funding

      Bornstein 2025, apheresis proof-of-concept

      Uncontrolled proof-of-concept case series · 21 patient eluate samples

      Part NIH (R21 MH126405) but several authors affiliated with commercial apheresis providers (INUS, TKM, Transmedac).

      Limits: Detects particles in discarded eluate only; no tissue-burden reduction; no clinical outcome.

    • Industry-funded

      Weinstein 2026, plasma exchange (peer-reviewed)

      Sponsor-affiliated clinical case series (no health outcomes) · 114 patients / 174 procedures

      Authors affiliated with Circulate Health, which sells plasma-exchange services.

      Limits: Lowers circulating counts only, not tissue burden; no clinical benefit; reduction obscured by plastic leaching from the apheresis tubing.

  • IV therapyNAD+ IV Unsupported
    • Funding unknown

      2026 PRISMA systematic review, NAD+ for anti-aging/wellness (Gallagher & Emmanuel, Ageing Res Rev)

      PRISMA systematic review · 113 studies screened (33 human, 80 rodent); no eligible IV/IM outcomes trials found

      Funding not displayed; lead author's disclosed commercial tie is to an aesthetics clinic that does NOT sell NAD+ products, so the review leans independent of NAD+ sellers. Treat funding as unverified.

      Limits: Synthesis, not a primary trial; full text could not be fetched first-party (verified via abstract and PubMed PMID 41655607). Conclusion of 'limited/no controlled IV outcomes evidence' is load-bearing and corroborated by the seller-side RCT.

    • Industry-funded

      Only IV NAD+ RCT, ChromaDex/Niagen acute pilot (2024 preprint)

      Randomized, double-blind, placebo-controlled pilot, acute blood-NAD+ and safety only · Up to ~53 healthy adults across two phases (37 in phase 1 + 16 in phase 2)

      Conducted by ChromaDex (now Niagen Bioscience) on its own competing NR-based IV; several authors are ChromaDex employees; sponsored via Franklin Health Research.

      Limits: Unrefereed preprint; measured only acute blood NAD+ and safety, no aging, energy, fatigue, or repair endpoint. NAD+ IV raised blood NAD+ LESS than the sponsor's NR IV.

    • Industry-funded

      2019 human pharmacokinetic pilot, 6-hour NAD+ infusion (Grant et al.)

      Pharmacokinetic pilot (controlled, non-randomized) · n=11 (8 NAD+, 3 saline), men aged 30–55

      Funded by NAD+ Research Inc.; co-author/director R. Mestayer is medical director of the Springfield Wellness Center, which sells IV NAD+ as a clinical therapy (disclosed COI).

      Limits: Tiny, short, acute; no efficacy/aging/energy endpoint. Plasma NAD+ flat for first 2h then +398%, with breakdown metabolites rising in parallel, degradation signature. No adverse events in this slow 6h protocol.

    • Industry-funded

      2026 Restore clinic retrospective, IV NAD+ vs NR tolerability (Reyna et al., Front Aging)

      Retrospective observational pilot (real-world EMR) · n=14 (NAD+ n=6, NR n=8); 500 mg/day x4 days

      Funded by Restore Hyper Wellness; NR product donated by Niagen Biosciences; multiple authors employed by Restore (bias acknowledged in the paper).

      Limits: No placebo; not designed to show efficacy. All 6 NAD+ patients had moderate-to-severe GI/cardiac side effects; mean NAD+ infusion ~97 min vs ~37 min for NR. Fatigue/QoL surveys were collected but not analyzed or reported as efficacy.

    • Independent

      Independent uncertainties review, NAD+ boosting strategies (Poljsak et al., Antioxidants 2022)

      Narrative review (independent) · na (evidence synthesis)

      Funded by the Slovenian Research Agency (P3-0388, P3-0019); authors declare no conflict of interest, the genuine non-commercial counterweight.

      Limits: Narrative, not systematic; but the central point holds, bioavailability, metabolism and tissue specificity of NAD+ boosters are unresolved, and there are no long-term human safety trials.

    • Mixed funding

      Clinical-evidence review, targeting NAD therapeutically (Radenkovic, Reason, Verdin, Pharmaceuticals 2020)

      Narrative clinical-evidence review · na

      No external funding reported; authors disclose commercial interests in NAD-related ventures (longevity institute, Repair Biotechnologies, Napa Therapeutics/Seneque).

      Limits: Narrative review; relevant point is that historical human IV NAD/NADH data are sparse and confined to disease populations, and infused NAD/NADH is likely cleaved before cellular uptake.

    • Independent

      NPR/KPBS reporting with named experts (Aubrey, 2026)

      Journalism with expert interviews · na

      Public-radio reporting; the key disconfirming quote ('no easy door,' 'very inefficient') is from Restore's own CSO and cuts against her commercial interest.

      Limits: Journalism, not a study; used for verbatim expert statements and documented pricing ($200–$1,000+/session), not as primary efficacy evidence.

    • Industry-funded

      ChromaDex/Niagen commercial disclosures (BioSpace, 2024)

      Company press release / financial disclosure (money trail only) · na

      Primary commercial source (the seller); used only to establish the verifiable money trail, never as evidence of efficacy.

      Limits: Marketing/financial material. Some figures (1,200+ clinics, 200+ Restore locations, ~$129.4M 2025 net sales) come from later ChromaDex releases, not this June-2024 page, see human-checks.

    • Industry-funded

      Martens 2018, healthy middle-aged/older adults

      Double-blind placebo-controlled crossover RCT, 6 weeks · n=24

      ChromaDex-linked program supplied NIAGEN; NR-supportive Seals lab.

      Limits: BP and aortic-stiffness signals exploratory only; authors called for future trials. Full disclosure sits in paywalled Nature text, not the PubMed page.

    • Industry-funded

      Elhassan 2019, aged men (median 75y)

      Double-blind placebo-controlled crossover RCT, 21 days · n=12

      ChromaDex provided NR/placebo; Brenner discloses patents, stock and advisory role.

      Limits: Blood NAD+ rose >2-fold but muscle NAD+ did not (p=0.22); all functional endpoints null (grip p=0.96); cytokine drop partly baseline-relative.

    • Mixed funding

      Remie 2020, healthy overweight/obese adults

      Double-blind placebo-controlled crossover RCT, 6 weeks · n=13

      Dutch Heart Foundation and EU Horizon 2020 funded; ChromaDex supplied NIAGEN with no design role.

      Limits: Insulin sensitivity and mitochondrial function null; only minor body-composition and acetylcarnitine shifts; 'no other metabolic health effects.'

    • Independent

      Brakedal 2022, NADPARK, newly diagnosed Parkinson's

      Double-blind placebo-controlled phase I RCT, 30 days · n=30

      Academic Norwegian trial, no industry sponsor.

      Limits: Raised cerebral NAD+ but only a subset showed 'mild' clinical change; explicitly hypothesis-generating, not disease modification or anti-aging proof.

    • Mixed funding

      Freeberg 2022, phase IIa blood-pressure trial (protocol only)

      Protocol for double-blind placebo-controlled phase IIa RCT, 3 months · n=94 planned

      NIH grants fund the work; ChromaDex supplied NIAGEN via material transfer agreement.

      Limits: Only the protocol is published; no primary results located, so the cardiovascular claim remains untested at the outcome level.

    • Independent

      VITAL

      Large RCT, primary prevention, 2x2 factorial vs vitamin D · n=25,871, median 5.3 yr, ~1 g/day EPA+DHA

      US NIH (NCI, NHLBI); product donated by Pronova/BASF; investigator-led.

      Limits: Null primary endpoint; the MI and subgroup signals (low fish-eaters, Black participants) are secondary, hypothesis-generating only.

    • Independent

      ASCEND

      Large RCT, primary prevention in diabetics · n=15,480, mean 7.4 yr, 840 mg/day (1 g capsule)

      Coordinated by Oxford; British Heart Foundation and others; not industry-controlled.

      Limits: Null result; olive-oil placebo. Tested only one low dose in diabetics.

    • Industry-funded

      REDUCE-IT

      Large RCT, high-risk, high-dose prescription EPA · n=8,179, median 4.9 yr, icosapent ethyl 4 g/day

      Funded by Amarin, which sells icosapent ethyl (Vascepa); direct interest in a positive result.

      Limits: Mineral-oil placebo was not biologically inert (raised LDL/apoB/CRP in controls), confounding the 25% benefit; never replicated.

    • Industry-funded

      STRENGTH

      Large RCT, high-dose omega-3 vs neutral placebo · n=13,078, median ~41 mo, omega-3 CA 4 g/day

      Funded by AstraZeneca; its own product (Epanova) failed, a null against the sponsor's interest.

      Limits: Stopped early for futility; same high dose as REDUCE-IT but null, plus an atrial-fibrillation harm signal.

    • Independent

      Cochrane review 2020

      Systematic review and meta-analysis · 86 RCTs, ~162,000 participants

      Cochrane / NIHR; no commercial sponsor; gold-standard synthesis.

      Limits: Small CV-mortality signal (RR 0.92) only moderate certainty and not matched by all-cause mortality.

    • Not applicable

      21 CFR 801.415, US federal ozone regulation

      Regulation / official statement · N/A

      US federal regulation.

      Limits: A regulatory position, not an outcome trial; sets the legal/scientific baseline that ozone has no recognised medical use.

    • Funding unknown

      Diabetic foot ulcer systematic review and meta-analysis (2024)

      Systematic review and meta-analysis · 11 studies, 960 patients

      Funding/COI not disclosed in the accessible record.

      Limits: Adjunct only; does not beat standard care for complete ulcer resolution; high heterogeneity. Risk-of-bias detail not confirmable from abstract.

    • Industry-funded

      Frontiers evidence and gap map of ozone as integrative medicine (2022)

      Evidence and gap map (umbrella mapping) · 26 systematic reviews, 55 outcomes

      Authors affiliated with advocacy bodies SOBOM and WFOT despite a 'no conflict' statement; favourable conclusions discounted.

      Limits: Only 9/26 reviews high quality; signal limited to pain/wounds; wound results not superior to conventional care.

    • Not applicable

      Cerebral gas embolism and ischemic stroke during oxygen-ozone therapy (2024)

      Case report · 1 patient

      Case report; no funding relevant.

      Limits: Single case; cannot establish incidence, but documents real embolic-stroke harm via patent foramen ovale during intradiscal ozone.

    • Independent

      Ozone therapy, encyclopedic synthesis of FDA/ACS positions and German 1975-1983 harm series

      Encyclopedic synthesis / harm compilation · German series: 6 deaths among multiple complications

      Tertiary source aggregating regulatory and peer-reviewed primary data.

      Limits: Harm data observational with no denominator; shows deaths and strokes occur but not their frequency.

    • Mixed funding

      Ocampo et al. 2016, Cell (cyclic OSKM)

      Preclinical: mouse plus in vitro human cells · LAKI 4F vs control cohorts; aged WT; cultured cells

      Salk/Izpisua Belmonte; NIH P30 CA014195. Izpisua Belmonte later joined Altos Labs.

      Limits: No human in vivo data; continuous OSKM caused teratomas, prompting cyclic dosing.

    • Mixed funding

      Lu et al. 2020, Nature (AAV-OSK, vision)

      Preclinical: mouse gene therapy plus in vitro human cells · Aged, optic-nerve-crush and glaucoma mouse cohorts; cultured human neurons

      NIH (R01 EY021526, R37 AG028730, R01 AG019719). Major COI: Sinclair holds equity/patents (Iduna/Life Biosciences).

      Limits: Single tissue; human work in vitro only; authors' commercial stakes.

    • Mixed funding

      Paine, Nguyen and Ocampo 2024, Aging Cell (review)

      Peer-reviewed narrative review · Review of preclinical literature and company pipelines

      Ocampo lab, Univ. of Lausanne; co-author also affiliated with EPITERNA SA, a longevity company (commercial tie).

      Limits: Narrative review, not new data; documents clock-vs-function disconnect and safety barriers.

    • Funding unknown

      Fight Aging! 2026 field summary

      Advocacy/longevity commentary · Not applicable

      Advocacy outlet summarizing peer-reviewed challenges.

      Limits: Weaker than a primary paper; corroborates that brief induction can still cause dedifferentiation/teratomas.

    • Independent

      Peng et al., Bioelectromagnetics 2020, bone-healing meta-analysis

      Systematic review & meta-analysis of RCTs · 22 studies, 1,468 participants (healing-rate figure from 1,131-patient subset)

      National Natural Science Foundation of China (Grant 81572231), a government funder; non-industry.

      Limits: Heterogeneous field parameters; very-low-quality evidence for healing time; positive healing-rate result drawn from a subset, not all 22 studies.

    • Independent

      EJPRM 2024, bone-fracture systematic review update

      Systematic review update (RCTs) · 3 RCTs, 197 patients (2014-2022)

      Authors declared no conflict of interest with any financial organization.

      Limits: Small pool; contradictory pain results; recommends against routine PEMF for acute fractures.

    • Not applicable

      FDA 510(k) clearance summary (secondary source)

      Regulatory clearance summary · n/a

      Commercial rehab retailer page, not a primary regulatory record.

      Limits: Confirms only the general framing (1979 clearance for non-union; adjunct post-op edema/pain). Specific device names and 510(k) numbers are NOT on this page and remain unverified against the FDA database.

    • Funding unknown

      Yang et al., J Rehabil Med 2019, knee-OA meta-analysis

      Systematic review & meta-analysis of RCTs · 8 RCTs, 421 patients

      No funding or conflict statement visible on the abstract.

      Limits: No significant pain or stiffness benefit; only physical function improved; parameter heterogeneity.

    • Funding unknown

      Vavken et al., J Rehabil Med 2009, knee-OA meta-analysis

      Meta-analysis of RCTs · 9 studies, 483 patients

      No funding or conflict statement visible on the abstract.

      Limits: Null on pain and stiffness; authors frame a functional adjuvant role positively, which sellers can overstate.

    • Funding unknown

      Zhongguo Gu Shang 2012, knee-OA systematic review (Cochrane-method)

      Systematic review (Cochrane methodology, not a formal Cochrane review) · 5 RCTs, 331 patients

      No funding or conflict statement visible.

      Limits: Little clinical benefit for pain; authors call for larger, more powerful trials; not authored by the Cochrane Collaboration.

    • Industry-funded

      HigherDOSE / Upgrade Labs, seller and biohacker longevity claims

      Marketing/blog (non-evidentiary) · n/a

      Commercial PEMF retailer/biohacking brand; direct conflict of interest.

      Limits: Source of the anti-aging messaging; contains no primary human longevity data; cited as claimant evidence, not scientific evidence.

    • Mixed funding

      Hickson 2019, D+Q in diabetic kidney disease

      Open-label single-arm Phase 1 pilot · n=9

      NIH plus non-profit foundations; key authors declared a financial interest and Mayo Clinic holds senolytic patents.

      Limits: Tiny, open-label, no placebo, 3-day dosing; D+Q combined; surrogate markers only, no aging endpoint.

    • Independent

      Justice 2019, D+Q in idiopathic pulmonary fibrosis

      Open-label single-arm first-in-human pilot · n=14

      States no pharmaceutical or for-profit funding (NIH plus foundations), but authors declared a financial interest and Mayo holds senolytic patents.

      Limits: Tiny, open-label, single-arm, 3 weeks; D+Q combined; no lung-function benefit; physical function only.

    • Not applicable

      NCT02848131 trial registry record

      Clinical trial registry record · early-phase (see Hickson n=9)

      Registry record; lead sponsor Mayo Clinic. Cited only to confirm the combination design.

      Limits: Not an outcome study; confirms intervention is dasatinib PLUS quercetin, not standalone quercetin.

    • Industry-funded

      Quercetin formulation pharmacokinetics in healthy adults

      Pharmacokinetic crossover pilot (human) · n=10

      Funded by Factors Group; compares a proprietary enhanced quercetin to standard quercetin.

      Limits: Reports relative absorption (7-15x) and 0.01 mg/mL solubility, not an absolute bioavailability figure; PK only.

  • DrugsRapamycin Unproven
    • Independent

      Harrison 2009, Nature (NIA ITP)

      Randomized controlled mouse lifespan study, 3 sites · ~1,901 UM-HET3 mice

      NIA grants + US Dept of Veterans Affairs; no industry.

      Limits: Mouse data only; says nothing directly about humans.

    • Independent

      Miller 2014, Aging Cell (NIA ITP)

      Randomized controlled mouse lifespan study · UM-HET3 mice, multiple dose arms

      NIA Interventions Testing Program; no industry sponsor.

      Limits: Mouse data only; effect dose- and sex-dependent; high exposure unlike tolerable human dosing.

    • Industry-funded

      Mannick 2014, Sci Transl Med

      Randomized placebo-controlled human trial (surrogate immune endpoint) · Elderly adults, flu-vaccine response endpoint

      Novartis-sponsored; authors Novartis-affiliated. Favourable surrogate, flagged.

      Limits: Surrogate endpoint (vaccine response) only; no healthspan or lifespan outcome.

    • Industry-funded

      resTORbio PROTECTOR 1, Phase 3 (2019)

      Phase 3 randomized human trial (clinical infection endpoint), failed · n=1,024 adults aged 65+

      Funded by resTORbio (Novartis-derived). Negative pivotal result; program halted.

      Limits: Endpoint was respiratory infection, not aging; OR 1.07, p=0.65.

    • Industry-funded

      PEARL trial, Aging (Albany NY) 2025

      Randomized double-blind placebo-controlled human trial (surrogate/self-report) · 125 randomized, 114 completed; aged 50–85

      Run by AgelessRx employees/shareholders (sells rapamycin); crowdfunded. Conflict flagged.

      Limits: Primary endpoint (visceral fat) missed, p=0.942; positive findings are secondary/self-reported; no mortality endpoint.

  • DietRaw milk Misleading
    • Independent

      Costard 2017, CDC outbreak risk model

      Quantitative outbreak risk model (national surveillance) · U.S. outbreak data, 2009-2014

      USDA grant plus Pennsylvania Agricultural Experiment Station; public/academic, no industry money. Finding is unfavorable to raw milk.

      Limits: 840x is a modeled per-unit rate ratio with a wide credible interval, not a direct headcount; the large-excess-risk conclusion is solid.

    • Industry-funded

      Mummah 2014, raw milk and lactose intolerance RCT

      Randomized, blinded crossover pilot trial · n=16 adults with confirmed lactose malabsorption

      Funded in part by the Weston A. Price Foundation, a pro-raw-milk group; a null result from a friendly funder is especially credible.

      Limits: Small pilot (n=16); blinded design and funder direction make the null persuasive.

    • Independent

      Macdonald 2011, pasteurization and milk vitamins meta-analysis

      Systematic review and meta-analysis · Pooled across multiple primary studies

      Canadian Institutes of Health Research (public); not industry-funded.

      Limits: Allergy-protection signal was observational and confounded; no support for cancer or lactose benefit.

    • Not applicable

      AAP fact-check on raw milk

      Professional society position / fact-check · Review of surveillance and literature

      Pediatric medical society; no commercial funding.

      Limits: Authoritative synthesis rather than a primary study.

    • Not applicable

      FDA 510(k) K103368, HairMax LaserComb (Lexington International), 2011

      Regulatory clearance record · n/a

      FDA database record; device made and sold by Lexington International, the seller.

      Limits: 510(k) substantial-equivalence pathway (Class II), reflects safety + a clinical signal, not drug-level proof of efficacy.

    • Independent

      Liu et al., Lasers Med Sci, 2019, LLLT for androgenetic alopecia

      Systematic review & meta-analysis of RCTs · 8 studies / 11 double-blind RCTs

      Taiwan Ministry of Science and Technology grant (MOST 107-2314-B-038-052); not industry-funded. Pooled trials still include manufacturer-run device studies.

      Limits: Pooled trials short (~16–26 wk); large effect (SMD 1.316, 95% CI 0.993–1.639) but device type did not change effectiveness; durability and severe-loss effect untested.

    • Independent

      Gupta et al., J Cosmet Laser Ther, 2018, LLLT for hair loss

      Systematic review · n/a (review)

      Independent review; explicitly calls for 'independent funding sources,' flagging that primary trials are sponsor-linked.

      Limits: Positive vs sham but warns results 'must be interpreted with caution'; primary trials small, short, often manufacturer-run.

    • Independent

      DermNet, low-dose laser therapy for hair loss

      Independent clinical reference · n/a

      Non-commercial dermatology education resource.

      Limits: Narrative summary; notes physicians disagree, trials criticised as not independent, anecdotal results 'disappointing.'

    • Industry-funded

      Wunsch & Matuschka, Photomed Laser Surg, 2014, red/NIR for skin

      Randomized controlled trial (industry-funded) · n=128 completers / ~15 wk

      'Fully funded by JK-Holding GmbH,' the device maker; PI 'mandated and remunerated by the sponsor'; co-author from JK-International.

      Limits: Sponsor-funded and sponsor-authored; surrogate skin endpoints; single trial; downgraded for conflict.

    • Industry-funded

      Couturaud et al., Skin Res Technol, 2023, Dior/Lucibel LED mask

      Uncontrolled before-after study (industry-authored) · n=20 / no control arm

      Authors include Lucibel's CEO and a Lucibel consultant, plus Parfum Christian Dior staff who sell the mask.

      Limits: No control/placebo; tiny n; large headline numbers (38% / 48%) likely overstate real-world effect; near-zero evidentiary weight.

    • Funding unknown

      Mota et al., Photobiomodul Photomed Laser Surg, 2023, periocular wrinkles

      Randomized split-face trial (no sham arm) · n=137 women / 4 wk

      Brazilian academic authorship; funding/COI not visible in the abstract and full text paywalled, unverified.

      Limits: Compares two active wavelengths with no true placebo; shows light does something, not superiority to sham; drew a published critical response.

    • Independent

      Carolina-Alves et al., Int J Mol Sci, 2024, PBM in dermatology

      Narrative review (independent) · n/a (review)

      Academic review; no manufacturer funding indicated (funding line not located on the page).

      Limits: Concludes skin studies 'not numerous,' protocols 'varied,' standardized recommendations 'lacking'; makes no longevity claim.

    • Independent

      Quirk & Whelan, Photobiomodul Photomed Laser Surg, 2020, CCO mechanism review

      Critical evidence review (mechanism) · n/a (review)

      Academic (Medical College of Wisconsin); supported by neurology endowments, no device-industry funding.

      Limits: A perspective within an active debate, not a final word; finds no reliable demonstration of a light effect on cytochrome c oxidase.

    • Mixed funding

      Baur/Sinclair, Nature 2006

      Animal study (mouse survival/healthspan) · Mice; small per-arm groups; standard vs high-calorie vs high-calorie+resveratrol

      NIH (NIA/NIGMS) funded; authors declared competing interests; Sinclair co-founded Sirtris to commercialize it.

      Limits: Survival benefit only vs overfed controls (HR 0.69); no extension vs standard diet (HR 1.03, p=0.88). Not healthy-lifespan extension.

    • Industry-funded

      Pacholec et al. (Pfizer), JBC 2010

      Biochemical / mechanistic study · In-vitro enzyme assays, NMR, SPR, ITC; plus high-fat-fed mouse confirmation for SRT1720

      Pfizer Global R&D; industry-funded but adverse to the resveratrol/Sirtris hypothesis.

      Limits: Mechanism study, not a clinical outcome trial; a minority allosteric/substrate-specific model is still debated.

    • Mixed funding

      Yoshino/Klein, Cell Metab 2012

      Randomized, double-blind, placebo-controlled trial (euglycemic clamp) · 29 nonobese postmenopausal women (15 resveratrol / 14 placebo); 75 mg/day; 12 weeks

      NIH + Longer Life Foundation; resveratrol supplied by DSM (one author employed there); a co-author on Sirtris SAB; still null.

      Limits: Small, short, single population; surrogate metabolic endpoints, not aging or mortality.

    • Independent

      Walle et al., Drug Metab Dispos 2004

      Human pharmacokinetics · 6 healthy volunteers; single 25 mg oral dose

      Academic (MUSC), NIH-supported; no supplement-industry sponsorship.

      Limits: Tiny n; single low dose; measures exposure, not clinical effect.

    • Independent

      Zhou et al., Front Physiol 2022

      Systematic review & meta-analysis of RCTs · 25 RCTs; 1,171 participants (593 resveratrol / 578 placebo)

      Chinese public/academic grants; no commercial conflicts reported.

      Limits: Fasting glucose non-significant (p=0.14); extreme heterogeneity (I2 81 to 94%); publication bias; no aging/longevity endpoint.

    • Independent

      Laukkanen KIHD sauna cohort (JAMA Intern Med 2015)

      Prospective observational cohort, ~21y · 2,315 Finnish men

      Non-U.S. government research support; no industry sponsor.

      Limits: Observational; residual confounding; one research group.

    • Independent

      Kunutsor/Laukkanen sauna cohort (BMC Medicine 2018)

      Prospective observational cohort · 1,688 men and women

      Finnish Foundation for Cardiovascular Research; non-commercial.

      Limits: Observational; overlaps same Finnish program.

    • Independent

      VITAL omega-3 RCT (NEJM 2019; PMID 30415637)

      Randomized double-blind placebo-controlled · 25,871 adults

      NIH/NHLBI funded; supplements donated but trial run independently.

      Limits: Replete population dilutes any deficiency benefit; null primary endpoint.

    • Mixed funding

      Omega-3 RCT update incl. REDUCE-IT (Cardiovasc Res 2023)

      Narrative update; REDUCE-IT was an RCT · REDUCE-IT: 8,179 high-risk patients

      Review by VITAL investigators; REDUCE-IT sponsored by Amarin.

      Limits: Pharmaceutical 4 g/day dose, secondary-prevention only; sponsor flag.

    • Independent

      Alumkal sulforaphane prostate trial (PMC4390425)

      Single-arm Phase II (no placebo) · 20 men

      Kuni Foundation, Prostate Cancer Foundation, NIH; non-commercial.

      Limits: Missed primary endpoint (1/20); surrogate marker; no longevity data.

    • Independent

      Laukkanen 2015 (KIHD, men), anchor

      Prospective observational cohort · 2,315 men

      Finnish Medical Foundation, Finnish Foundation for Cardiovascular Research, Finnish Cultural Foundation; no conflicts reported. No sauna-industry money.

      Limits: Men only; single baseline self-report; residual confounding possible.

    • Independent

      Laukkanen 2018 (KIHD, +women)

      Prospective observational cohort · 1,688 (867 women, 821 men)

      Finnish Foundation for Cardiovascular Research; no competing interests.

      Limits: Same cohort family, not independent; 181 CVD events; single baseline measure.

    • Independent

      Gravel 2023, mechanistic RCT (null)

      Randomized controlled trial · 41 (33 men, 8 women)

      Academic physiology lab (Canada); no sauna-industry sponsorship identified.

      Limits: Small, 8 weeks, surrogate endpoints; CAD patients; no hard outcome.

    • Independent

      Laukkanen 2017, dementia/Alzheimer's

      Prospective observational cohort · 2,315 men

      Finnish Foundation for Cardiovascular Research; no conflicts.

      Limits: Same KIHD men; reverse causation (prodromal disease) possible; coded outcomes.

    • Independent

      Kunutsor 2022, inflammation × sauna

      Prospective observational cohort · 2,575 men

      Finnish Foundation for Cardiovascular Research; no conflicts.

      Limits: Authors cite residual confounding, reverse causation, regression dilution.

  • DietSeed oils Unsupported
    • Mixed funding

      Mozaffarian 2010, PLoS Medicine

      Meta-analysis of 8 RCTs · 13,614 participants

      NIH/NHLBI and a Searle Scholar Award led the funding with no funder role; lead author disclosed broad food/pharma honoraria incl. Unilever.

      Limits: CHD endpoints only; older trials with varied PUFA sources.

    • Independent

      Hooper/Cochrane 2020, CD011737

      Cochrane meta-analysis of RCTs · 15 RCTs, ~56,675 participants

      Official NIHR/Cochrane review; no industry sponsorship of the review (funding not stated on the cited plain-language page).

      Limits: Benefit is for cardiovascular events; little or no effect on all-cause or CVD mortality.

    • Funding unknown

      Johnson & Fritsche 2012, J Acad Nutr Diet

      Systematic review of RCTs · 15 RCTs

      Funding/COI not stated on the NCBI/DARE abstract reviewed; second author is an academic lipid-immunology researcher.

      Limits: Healthy adults, modest duration; measures blood markers, not disease endpoints.

    • Independent

      Rett & Whelan 2011, Nutr Metab (Lond)

      Systematic review of human studies · 36 studies

      Authors declared no competing interests; public/academic funding (USDA NC1039, Tennessee Agricultural Experiment Station).

      Limits: Western-diet adults; surrogate biomarker (arachidonic acid), not clinical outcomes.

    • Mixed funding

      Marklund 2019, Circulation

      Pooled biomarker cohort analysis · 30 cohorts, 68,659 participants

      FLAG: two co-authors reported Unilever research support for this work; senior author disclosed multiple funders. Result favourable to PUFA.

      Limits: Observational biomarker associations, not randomized; residual confounding possible.

    • Independent

      Grootveld 2018, editorial (PMC6131264)

      Review/editorial on heated-oil oxidation · Narrative review

      Only a university PhD fee-waiver scholarship; authors declared no conflicts of interest.

      Limits: Concerns reheated/reused oil; human outcome evidence limited, largely animal/mechanistic.

    • Industry-funded

      SELECT (Lincoff 2023)

      RCT, double-blind, placebo-controlled (41 countries) · 17,604 adults, obesity + established CVD, no diabetes

      Funded and run by Novo Nordisk; sponsor-favourable result, published in NEJM.

      Limits: High-risk population only; does not test aging or lifespan.

    • Industry-funded

      STEP 1 (Wilding 2021)

      RCT, double-blind, placebo-controlled, 68 weeks · 1,961 adults with obesity, no diabetes

      Funded by Novo Nordisk.

      Limits: Endpoint is weight, not aging; GI and gallbladder adverse events noted.

    • Industry-funded

      FLOW (Perkovic 2024)

      RCT, double-blind, placebo-controlled · 3,533 adults, type 2 diabetes + CKD

      Funded by Novo Nordisk; stopped early for efficacy.

      Limits: Diabetic CKD only; not an aging endpoint.

    • Industry-funded

      STEP 1 DXA substudy (2021)

      Exploratory DXA body-composition substudy · 140 (95 semaglutide, 45 placebo)

      Substudy of Novo Nordisk-funded STEP 1.

      Limits: Small; lean mass fell 9.7% (~40% of mass lost), though lean rose as a proportion of total body mass.

    • Industry-funded

      STEP 1 withdrawal extension (Wilding 2022)

      Off-treatment extension of an RCT · 327 participants

      Novo Nordisk-funded STEP 1 extension.

      Limits: ~two-thirds of lost weight (11.6 pts) regained within a year; cardiometabolic gains reverted toward baseline.

    • Independent

      Baker / van Deursen, Nature 2016 (genetic ablation)

      Animal (transgenic mice, INK-ATTAC senescent-cell ablation) · Wild-type and transgenic mouse cohorts

      NIH/foundation funded (Mayo Clinic); Mayo holds senolytic patents and senior authors have related interests.

      Limits: Mouse model; genetic, not a drug; does not predict human anti-aging benefit.

    • Independent

      Xu / Kirkland, Nature Medicine 2018 (D+Q in old mice)

      Animal (naturally aged mice, oral senolytics) · Multiple mouse cohorts, late-life dosing

      NIH funded; stated COI: patents on senolytic drugs (PCT/US2016/041646) held by Mayo Clinic; Kirkland, Tchkonia, Xu disclose interests.

      Limits: Mouse lifespan endpoint; translation to humans unproven.

    • Independent

      Hickson, EBioMedicine 2019 (diabetic kidney disease)

      Human open-label pilot (single arm, biomarker endpoint) · 9 patients, mean age 68.7

      NIH/foundation funded; Mayo-affiliated authors with disclosed senolytic patent interests.

      Limits: No placebo, n=9, 3-day dosing; measures tissue senescence markers, not aging or lifespan.

    • Independent

      Justice, EBioMedicine 2019 (pulmonary fibrosis)

      Human open-label pilot (feasibility, no control arm) · 14 patients with idiopathic pulmonary fibrosis

      NIH (NIA/NCATS) funded; Mayo-affiliated authors with disclosed patent interests.

      Limits: Uncontrolled, underpowered; disease-specific function signals, not an aging endpoint.

    • Industry-funded

      Unity UBX0101 Phase 2, 2020 (knee osteoarthritis)

      Human randomized double-blind placebo-controlled Phase 2 (null result) · 183 patients, moderate-to-severe knee OA

      Sponsored by Unity Biotechnology; negative result reported against commercial interest, which strengthens its credibility.

      Limits: Disease (pain) endpoint, single intra-articular dose; failed primary endpoint, program discontinued.

    • Independent

      Eisenberg 2009, Nature Cell Biology (mechanism)

      Experimental, multiple model organisms (yeast, flies, worms, human cells in vitro) · Model organisms / cell lines

      2009 basic-science paper predating the Longevity Labs venture.

      Limits: No human in vivo data; establishes mechanism, not clinical benefit.

    • Mixed funding

      Eisenberg 2016, Nature Medicine (mouse + Bruneck)

      Animal experimental (mouse) plus human observational dietary epidemiology · Mice plus ~829 humans (Bruneck dietary data)

      Madeo/Eisenberg later founded or advised TLL; human arm is diet, not supplementation.

      Limits: Human arm observational only; supplement benefit not tested in people.

    • Mixed funding

      SmartAge RCT, Schwarz 2022, JAMA Network Open

      Randomized, double-blind, placebo-controlled trial (phase 2b) · n=100 (89 completed), 12 months

      Public funders (BMBF, DFG, BIH) but TLL funded extract development; Madeo/Sigrist/Eisenberg held equity/advisory roles, Stekovic was TLL CEO, Madeo holds a pending patent.

      Limits: Null on primary endpoint despite favorable conflicts; low dose (0.9 mg/d) cannot fully exclude effect at other doses.

    • Mixed funding

      Wirth 2018, Cortex (pilot)

      Randomized, double-blind, placebo-controlled pilot (phase 2a) · n=30, 3 months

      Madeo among authors; extract supplied for the trial.

      Limits: Tiny n, short duration; authors called for a confirmatory trial that (SmartAge) then failed to confirm.

    • Mixed funding

      Kiechl 2018, AJCN (Bruneck mortality)

      Prospective observational cohort (dietary intake) · ~829 (Bruneck), ~20-yr follow-up, validated in SAPHIR

      Overlapping author group (Eisenberg) with the TLL ecosystem.

      Limits: Diet, not supplementation; association not causation; healthy-user/healthy-diet confounding.

    • Funding unknown

      Mini-review of cognitive RCTs, PPCR 2023

      Mini-review / evidence synthesis of RCTs · 3 RCTs included (from 1726 screened), ages 60-96

      Funding/COI not stated; used to characterize the evidence base, not as an efficacy source.

      Limits: Few small heterogeneous trials, low doses; 2 positive at 3 months, 1 null at 12 months.

  • DrugsStatins Misleading
    • Industry-funded

      JUPITER RCT (rosuvastatin, primary prevention)

      Randomised double-blind placebo-controlled trial · 17,802 adults

      Funded by AstraZeneca, maker of rosuvastatin; lead author named on the hs-CRP patent used to select patients. Flagged because favourable to sponsor; verdict does not rest on it.

      Limits: Funding/COI widely documented but not confirmable from the loaded abstract; trial stopped early, which can exaggerate effect.

    • Independent

      SAMSON n-of-1 nocebo trial

      Double-blind n-of-1 randomised crossover · 60 previously intolerant patients

      British Heart Foundation; no manufacturer involvement.

      Limits: Small sample; exact symptom-score figures sit in a paywalled NEJM correspondence and are not independently verified.

    • Independent

      Sattar diabetes meta-analysis

      Meta-analysis of RCTs (harm outcome) · 13 trials, 91,140 participants

      Stated funding: None; investigator-initiated.

      Limits: Quantifies one harm only; reports a real but small risk, bounded and outweighed by vascular benefit in at-risk groups.

    • Not applicable

      BMJ correction of the '18-20%' side-effect figure

      Journal correction (documents claimant error) · n/a

      Retraction Watch summary of a BMJ editorial correction.

      Limits: Documents withdrawal of the 18-20% figure as a misread observational study; does NOT itself state any corrected '~9%' replacement rate.

    • Independent

      Kuriyan et al., NEJM 2017 (blindness after eye injections)

      Case series (3 patients, 6 eyes) · 3 patients

      NIH/National Eye Institute grant P30 EY014801; no industry funding. Academic ophthalmologists reporting harm.

      Limits: Case series; documents that severe harm can occur but cannot quantify population incidence.

    • Independent

      El Assaad et al., scoping review of stem cells for anti-aging, World J Exp Med 2024

      Scoping review · Literature review; no human anti-aging RCTs identified

      Academic review; authors declare no conflict of interest and no external funding.

      Limits: Scoping (not systematic) review; confirms absence of rigorous human evidence rather than testing efficacy.

    • Independent

      Liu et al., MSCs/EVs as longevity tools, Biogerontology 2025

      Narrative review (preclinical-focused) · Animal data (rodent)

      US-Israel Binational Science Foundation and Israeli government/academy grants; authors declare no competing interests. Non-industry.

      Limits: Effects limited to rodents; human extrapolation called 'mostly speculative.' Reviews preclinical work, not human outcomes.

    • Independent

      ISSCR Guide to Stem Cell Treatments (patient handbook)

      Professional society guidance / consensus · n/a

      Non-profit scientific society; patient-education guidance, not industry-funded.

      Limits: Guidance, not primary data; reflects expert consensus against marketed clinic offerings.

  • DietSugar Mixed
    • Independent

      Te Morenga, Mallard & Mann (BMJ 2013)

      Systematic review & meta-analysis (30 RCTs + 38 cohorts) · 68 studies; thousands of participants

      Academic/public-health authors (Mann advised WHO); no industry funding. The neutral cross-check.

      Limits: Cohort component is observational; many RCTs short.

    • Independent

      WHO sugars guideline (2015)

      Evidence-based guideline (GRADE) · Synthesis of systematic reviews

      World Health Organization; no commercial funding.

      Limits: Guideline, not new trial data; no toxin framing used.

    • Independent

      Malik & Hu (Nutrients 2019)

      Evidence review of meta-analyses & cohorts · 17+ cohorts for diabetes

      No external funding, but senior author does pro bono anti-SSB litigation support (CSPI); flagged.

      Limits: Largely observational; residual confounding possible.

    • Industry-funded

      Khan & Sievenpiper (Eur J Nutr 2016)

      Review of meta-analyses of controlled feeding trials · Isocaloric & hypercaloric fructose trials

      Authors disclose Coca-Cola, Dr Pepper Snapple, Canadian Sugar Institute and other food-industry ties; conflict flagged.

      Limits: Industry-funded; feeding trials short and small.

    • Not applicable

      Lustig, "Sugar: The Bitter Truth" (2009)

      Claimant source (lecture/advocacy) · N/A

      Advocacy source cited to represent the claim, not as evidence.

      Limits: Not a study; rhetorical framing under review.

    • Independent

      Egner/Kensler Qidong broccoli-sprout detox RCT

      RCT, surrogate biomarker · n=291, 12 weeks

      Academic/government (Johns Hopkins, NIH/NCI); no supplement-seller sponsorship.

      Limits: Endpoint is a urinary detox biomarker, not cancer or any clinical outcome.

    • Independent

      Zimmerman autism replication

      RCT, replication · n=45, 15 weeks

      U.S. Department of Defense; independent of industry.

      Limits: Null on primary autism outcome; only one secondary scale improved.

    • Independent

      Alumkal recurrent-prostate phase II

      Phase II single-arm · n=20, up to 20 weeks

      Academic/NIH (OHSU); funding consistent with affiliation, not re-verified from disclosure.

      Limits: Primary PSA endpoint failed (1/20); only a secondary doubling-time signal.

    • Mixed funding

      Axelsson type-2-diabetes RCT

      RCT, surrogate metabolic · n≈97, 12 weeks

      Lund University; two authors are inventors on sulforaphane patent applications.

      Limits: Glucose/HbA1c benefit restricted to an obese, dysregulated subgroup.

    • Not applicable

      Wikipedia clinical-evidence summary

      Tertiary evidence summary · n/a

      Non-commercial reference summarizing secondary sources.

      Limits: States no good clinical evidence of benefit despite extensive animal data.

    • Independent

      Matta et al., JAMA 2019, FDA maximal-use absorption RCT

      Randomized clinical trial (pharmacokinetic) · 24 healthy adults

      Conducted and funded by the U.S. FDA; no commercial sponsor, no conflicts reported.

      Limits: Plasma-level study only; measures absorption, never designed to detect clinical harm.

    • Independent

      Matta et al., JAMA 2020, FDA maximal-use absorption RCT

      Randomized clinical trial (pharmacokinetic) · 48 healthy adults

      FDA-funded and authored; conflicts 'none reported.'

      Limits: Single-application PK design; shows absorption, not toxicity.

    • Independent

      Green et al., JCO 2011, Nambour melanoma follow-up

      Randomized controlled trial (long-term follow-up) · 1,621 adults

      Academic/government-supported Australian trial; not industry-funded.

      Limits: Total-melanoma CI marginal (upper 1.02); high-UV Australian setting may limit generalizability.

    • Independent

      Green et al., Lancet 1999, Nambour base RCT (squamous-cell)

      Randomized controlled trial (2x2 factorial) · 1,621 adults

      Academic/government-supported; not industry-funded.

      Limits: 4.5-year endpoints; verified via PubMed as cited journal URL was inaccessible.

    • Independent

      Harvard Health, the science of sunscreen (citing JAAD analysis)

      Expert review citing toxicology analysis · N/A (review)

      Academic medical publisher; no industry sponsorship indicated.

      Limits: Secondary source; relays the 277-year exposure-margin estimate from underlying JAAD work.

    • Independent

      Singh et al., Science 2023 (foundational, multi-species)

      Animal interventional lifespan + human cross-sectional association · Mice, ~15 rhesus monkeys, worms/yeast; human EPIC-Norfolk n=11,966

      NIH (NIA/NHLBI/NICHD) and Wellcome Trust; Columbia filed a provisional patent (inventor V.K. Yadav), a minor flag.

      Limits: Lifespan effect is animal-only; human data associational, no outcome trial.

    • Independent

      Fernandez et al., Science 2025 (de Cabo, NIH): is taurine an aging biomarker?

      Longitudinal biomarker reanalysis (humans, monkeys, mice) · Baltimore Longitudinal Study of Aging (ages 26-100), monkeys, mice

      NIH National Institute on Aging intramural; no commercial conflicts.

      Limits: Biomarker analysis, not an intervention; sex-specific patterns.

    • Independent

      Marcangeli et al., Aging Cell 2025: evidence against taurine deficiency in humans

      Cross-sectional human phenotyping · 137 men, aged 20-93

      Canadian Institutes of Health Research, NSERC, FRQS; no conflicts.

      Limits: Men only; cross-sectional, not interventional.

    • Independent

      Tzang et al., Nutrition & Diabetes 2024: taurine and metabolic syndrome

      Systematic review and meta-analysis of RCTs (surrogate markers) · 25 RCTs, n=1,024; 0.5-6 g/day; 5-365 days

      National Taiwan University Hospital and Taiwan science ministry; no conflicts.

      Limits: Short-term surrogate markers (BP, glucose, triglycerides), not lifespan/healthspan; small heterogeneous trials.

  • PeptidesTB-500 Unproven
    • Industry-funded

      SEER-1: RGN-259 (Tb4) eye drops, neurotrophic keratopathy

      Randomized, placebo-controlled, double-masked Phase 3 RCT · n=18 (10 active, 8 placebo)

      Funded by ReGenTree, LLC; authors Kleinman and Kang employed by ReGenTree, Sung by parent HLB Therapeutics.

      Limits: Tiny n. Primary endpoint (complete healing at Day 29, 60% vs 12.5%) was NOT significant (p=0.0656). Eye drop, not injectable, not a recovery use.

    • Independent

      BSCG regulatory and safety review of TB-500

      Expert review (independent anti-doping lab) · N/A (review)

      Independent third-party testing body founded by Olympic drug-testing expert Don Catlin; no stake in selling TB-500.

      Limits: Narrative review, not primary data. Synthesises regulatory status, contamination risk and the thin trial record.

    • Funding unknown

      Xing et al., review of thymosin beta-4 function

      Narrative review (preclinical and early clinical) · N/A (review)

      Academic review; funding not stated on the fetched content.

      Limits: Confirms human evidence is limited to Phase 1/2; no Phase 3 for tissue repair. Oncologic signal is mechanistic, not proof of harm in users.

    • Industry-funded

      Topical Tb4 Phase 2 venous-stasis ulcer trial

      Phase 2 double-blind placebo-controlled RCT (topical) · n~73

      Sponsor (RegeneRx)-developed compound. Cited via BSCG summary, not the primary publication.

      Limits: Topical pharmaceutical Tb4 for ulcers, not injectable TB-500 for recovery. Overall healing not conclusively superior to control.

    • Funding unknown

      Ho et al., LC-MS detection of TB-500 in horses

      Analytical/pharmacokinetic method paper (equine) · Single 10 mg dose administered

      Hong Kong Jockey Club racing-laboratory authors; anti-doping detection context, not a product sponsor.

      Limits: Establishes identity and equine doping use, not human efficacy or safety. Source does not state the number of horses dosed.

    • Independent

      Martin-Ruiz 2014, Int J Epidemiol: 10-lab blinded reproducibility

      International inter-laboratory reproducibility study · 10 labs; 12 distinct DNA samples sent for analysis across two blinded rounds

      UK MRC, Swedish Cancer Society, British Heart Foundation; 'Conflict of interest: None declared.'

      Limits: Tests lab reproducibility, not clinical outcomes; reports CVs across methods, not personal accuracy.

    • Independent

      Kim 2011, PLoS ONE: monochrome-multiplex qPCR test-retest

      Single-lab test-retest reliability study · 27 women aged 35-74; 186 visit-based comparisons over 9 months

      NIH/NIEHS Intramural Program; no competing interests.

      Limits: Small, single lab; actually found acceptable short-term reliability under controlled conditions.

    • Not applicable

      Armanios interview, Science News 2018

      Expert interview (science journalism) · Expert commentary; no original cohort

      Journalist quoting an independent academic researcher.

      Limits: Commentary, not primary data; Swedish/22-year figures belong to source 4, not this interview.

    • Not applicable

      Lewis 2018, PLOS DNA Science blog

      Expert commentary (science journalism) · Cites experts and a 100-donor reproducibility example

      PLOS science blog corroborating primary reliability data.

      Limits: Secondary commentary; anchors to peer-reviewed studies above.

    • Industry-funded

      Salvador 2016, Rejuvenation Research: TA-65 RCT

      Randomized, double-blind, placebo-controlled trial (sponsor-conflicted) · 117 enrolled, 97 completed; 12 months; 3 arms

      Funded by T.A. Sciences (maker of TA-65); 2 author-employees + 3 author-consultants.

      Limits: Shows a +530 bp length gain, not that consumer tests measure biological age; conflicted authorship.

    • Industry-funded

      SURMOUNT-1 (obesity)

      Phase 3 RCT, double-blind, placebo-controlled, 72 wk · 2,539

      Funded by Eli Lilly; several authors are Lilly employees/consultants.

      Limits: Endpoint is weight, not lifespan; topline ITT ~20.9% at 15 mg (per-protocol estimand slightly higher).

    • Industry-funded

      SURPASS-2 (type 2 diabetes)

      Phase 3 RCT, open-label, active comparator, 40 wk · 1,879

      Funded by Eli Lilly; comparator was semaglutide (Novo).

      Limits: Surrogate metabolic endpoints (HbA1c, weight), not lifespan.

    • Industry-funded

      SURPASS-CVOT (CV outcomes)

      Phase 3 RCT, active comparator, ~4-yr follow-up · 13,299

      Eli Lilly makes both tirzepatide and comparator dulaglutide.

      Limits: Noninferior not superior for MACE; mortality benefit largely noncardiovascular; high-risk diabetics only.

    • Industry-funded

      SUMMIT (HFpEF + obesity)

      Phase 3 RCT, double-blind, placebo-controlled · 731

      Explicitly funded by Eli Lilly.

      Limits: Disease-specific HF outcome, not a general aging/lifespan endpoint.

    • Industry-funded

      SURMOUNT-1 body composition

      Prespecified DXA substudy of a Phase 3 RCT · 2,539

      Eli Lilly-funded; authors include Lilly affiliates.

      Limits: ~75% fat / 25% lean loss; absolute lean mass fell, basis for the muscle-loss concern.

    • Industry-funded

      Chinnappan/Physta 12-wk RCT, hypogonadal men

      RCT (double-blind, placebo-controlled) · n=105, 12 weeks

      Funded by Biotropics Malaysia, maker of Physta; two authors were company employees.

      Limits: Total T rose vs placebo but free T did not; maker-funded; only low-T older men.

    • Industry-funded

      Talbott tongkat ali stress RCT

      RCT (double-blind, placebo-controlled) · n=63, 4 weeks

      Funded by Biotropics Malaysia; authors tied to tongkat-ali and nutrition firms.

      Limits: Short, mixed-sex, maker-funded; stressed adults only.

    • Independent

      Yakubu Fadogia aphrodisiac (rats)

      Preclinical animal (rat) · Male rats, days 1 to 5

      Academic (University of Ilorin); no industry sponsor.

      Limits: Rodent-only, short; no human evidence; rat count unspecified.

    • Independent

      Yakubu Fadogia testicular toxicity (rats)

      Preclinical animal (rat) toxicity · Male rats, 28 days

      Academic (University of Ilorin); no industry sponsor.

      Limits: Single group, small; establishes a signal, not a human risk figure.

    • Not applicable

      Read Andrew Huberman (claimant summary)

      Promotional/claimant documentation · n/a (anecdote)

      Secondary Substack summary with affiliate links; documents claim, not efficacy.

      Limits: Some attributed figures are not actually on this page; not evidence.

  • HormonesTRT Misleading
    • Independent

      Testosterone Trials (Snyder), NEJM 2016

      Coordinated set of RCTs (double-blind, placebo-controlled) · n=790, age 65+ / 1 yr

      NIH (National Institute on Aging, NIDDK); AbbVie donated drug and placebo. The independently funded efficacy trial, and the one that returned the null vitality result.

      Limits: Enrolled only men with low T plus symptoms (cannot speak to normal-T "anti-aging" use); 1-year duration; benefit largely confined to sexual function.

    • Industry-funded

      TRAVERSE (Lincoff), NEJM 2023

      RCT, cardiovascular non-inferiority (FDA-mandated) · n=5,246, age 45–80 / ~22 mo

      Funded by a consortium of testosterone manufacturers led by AbbVie (with Endo, Allergan, Upsher-Smith, Acerus/Aytu). The reassuring safety result carries this conflict-of-interest flag.

      Limits: Diagnosed hypogonadism only; powered for MACE non-inferiority, not for the AF/VTE/AKI signals it nonetheless showed; monitored transdermal gel, so may not generalise to unmonitored or high-dose injectable use.

    • Independent

      Endocrine Society guideline (Bhasin), JCEM 2018

      Clinical practice guideline · Evidence synthesis

      Endocrine Society professional guideline.

      Limits: Recommends TRT only for documented hypogonadism; recommends against routine prescribing to all men 65+ with low T; does not address, let alone endorse, anti-aging use in normal-T men.

    • Industry-funded

      TRAVERSE prostate substudy (Bhasin), JAMA Netw Open 2023

      RCT substudy (prostate safety) · n=5,204 / ~22 mo

      Same AbbVie-led manufacturer consortium as TRAVERSE, favourable result, COI flag.

      Limits: Monitored hypogonadal men only; the high-grade prostate-cancer point estimate was numerically above 1 (HR 1.62) though non-significant on a wide interval; ~22-month follow-up cannot address long-latency cancer risk.

    • Independent

      TOM trial (Basaria), NEJM 2010

      RCT, stopped early · n=209, frail older men

      NIH-funded. The independent trial that produced the cautionary signal behind the FDA's 2015 warnings.

      Limits: Stopped early for excess cardiovascular events; small; enrolled frail older men with mobility limits and high rates of pre-existing cardiac disease, so the signal may not generalise to healthier men.

    • Independent

      Prostate-safety network meta-analysis, Front Endocrinol 2022

      Bayesian network meta-analysis (30 RCTs + 5 cohorts) · ~7,740 participants

      Academic (Chinese government/NSFC grants).

      Limits: Pools cohort studies alongside RCTs; reassuring on prostate cancer (RR 0.62) but in monitored low-T/hypogonadal men, not normal-T anti-aging users.

    • Industry-funded

      Andreux 2019, Nature Metabolism (first-in-human)

      RCT, double-blind, placebo-controlled safety/PK; single dose + 4 weeks, ~n=36 · ~36 healthy sedentary elderly

      Funded by Amazentis SA; authors include Amazentis founders/employees (Rinsch, Auwerx, Aebischer, Singh).

      Limits: Primary endpoint was safety; no strength or function tested; 4 weeks; small.

    • Industry-funded

      Liu 2022, ENERGIZE, JAMA Network Open

      RCT, double-blind, placebo-controlled; 1000 mg/day, 4 months · 66 older adults (33 UA / 33 placebo; mean ~72)

      "This research was supported by Amazentis SA." D'Amico, Singh employees; Rinsch founder/CEO/board; Aebischer chair/shareholder.

      Limits: Both co-primary endpoints (6-min walk, max ATP) missed; endurance secondary, attenuated by 4 months; ~76% female, 100% White.

    • Industry-funded

      Singh 2022, ATLAS, Cell Reports Medicine

      RCT, double-blind, placebo-controlled, 3-arm; 500/1000 mg, 4 months · 88 untrained overweight middle-aged (29/29/30)

      "This study was funded by Amazentis SA." Multiple authors employees/board; Auwerx and Aebischer on Scientific Advisory Board of the sponsor.

      Limits: Primary peak-power endpoint missed; 12% gain is secondary torque; grip/quadriceps NS; 6MWT/VO2/gait within-group, NS vs placebo.

    • Independent

      ADDF / Cognitive Vitality evidence review (Oct 2024)

      Independent evidence review (no primary participants) · Review

      ADDF/Cognitive Vitality is a non-industry reviewer.

      Limits: Narrative review; confirms animal-only lifespan data and both missed primary endpoints; "good safety but limited benefits in short term studies so far."

    • Mixed funding

      VITAL (Manson 2019, NEJM)

      Double-blind RCT, 2x2 factorial, 2000 IU/day · n=25,871; median 5.3 yr

      U.S. NIH (NCI/NHLBI; grants U01/R01 CA138962). Capsules donated by Pharmavite (vitamin D) and Pronova BioPharma (omega-3); trial investigator-designed and -analysed.

      Limits: Participants not selected for deficiency; a post-hoc normal-BMI cancer signal (HR 0.76) is hypothesis-generating, not a primary result.

    • Independent

      D-Health (Neale 2022, Lancet Diab Endocrinol)

      Double-blind RCT, 60,000 IU/month; mortality primary · n=21,315; ~5 yr

      Australian NHMRC; no commercial sponsor of design or analysis.

      Limits: All-cause mortality null (HR 1.04); cancer mortality numerically higher (HR 1.15, 0.96–1.39), reaching HR 1.24 (1.01–1.54) excluding the first 2 years, a possible adverse signal, not benefit. Monthly bolus dosing.

    • Mixed funding

      VITAL fracture ancillary (LeBoff 2022, NEJM)

      Ancillary analysis of VITAL RCT · n=25,871

      U.S. NIAMS (R01 AR060574, AR070854, AR059775) plus parent VITAL NIH funders.

      Limits: Adults not recruited for deficiency, low bone mass, or osteoporosis; total HR 0.98, nonvertebral 0.97, hip 1.01.

    • Independent

      Zhang 2019 (BMJ meta-analysis)

      Systematic review/meta-analysis of RCTs · 52 RCTs; n=75,454

      National Natural Science Foundation of China; National Key R&D Program of China. No supplement-industry funding.

      Limits: All-cause mortality null (RR 0.98, 0.95–1.02); a significant cancer-mortality reduction (RR 0.84, 0.74–0.95) is a contested secondary signal not reproduced, and arguably reversed, in D-Health.

    • Not applicable

      Cummings & Rosen 2022 (NEJM editorial)

      Invited editorial synthesizing the trial evidence · No sample

      Independent academic clinicians (UCSF; Maine Medical Center / Tufts).

      Limits: Opinion synthesis, not new data; PubMed page carries no abstract.

    • Independent

      Jolliffe 2021 (Lancet Diab Endocrinol)

      Systematic review/meta-analysis (incl. IPD) · 46 RCTs; 48,488 in primary analysis

      Paper's funding statement reads 'None.'

      Limits: Protection against acute respiratory infection modest (OR 0.92, 0.86–0.99) and concentrated in daily low-dose regimens (400–1000 IU); no significant effect by baseline 25(OH)D subgroup. A narrow benefit, not validation of the longevity claim.

    • Industry-funded

      Knapen 2015, MK-7 arterial stiffness RCT

      3-yr double-blind RCT, surrogate endpoint, n=244 · n=244

      VitaK/Maastricht, tied to NattoPharma; used branded MenaQ7.

      Limits: Surrogate (arterial stiffness), not clinical events; manufacturer link.

    • Mixed funding

      AVADEC (Diederichsen 2022), aortic valve calcification

      2-yr double-blind RCT, hard imaging endpoint, n=365 · n=365

      Independent Danish academic design; ingredient from Kappa Bioscience (K2Vital), not MenaQ7.

      Limits: Null primary outcome; men only; coronary-calcium hint was exploratory.

    • Funding unknown

      Rotterdam Study (Geleijnse 2004), dietary K2 and CHD

      Prospective observational cohort, n=4807 · n=4807

      Academic Dutch cohort; original funding not confirmed from opened source.

      Limits: Dietary intake, confounding-prone, not causal; not replicated cleanly.

    • Independent

      Ma 2022, K2 and postmenopausal osteoporosis

      Systematic review/meta-analysis, 16 RCTs, 6425 · 16 RCTs; 6425

      Chinese academic grants; independent of K2 supplement industry.

      Limits: K2-alone not significant for BMD; fracture signal driven by Japanese MK-4.

    • Independent

      Cochrane review, muscle soreness/recovery

      Systematic review (4 RCTs) · 64 participants

      Cochrane; no commercial sponsor.

      Limits: Very-low certainty; mostly young men; no adverse-event surveillance.

    • Funding unknown

      He et al., inflammation meta-analysis

      Meta-analysis of RCTs · 11 RCTs, n=274

      Authors declare no competing interests; funding not stated.

      Limits: Small, heterogeneous, short-term; surrogate markers; abstract conclusion more bullish than data warrant.

    • Independent

      Stanek et al., ankylosing spondylitis RCT

      Small RCT · 32 men

      Medical University of Silesia grants; no conflicts declared.

      Limits: Tiny sample; 10-day window; no long-term follow-up; IL-6 unchanged.

    • Independent

      J Evid Based Med indexed abstract of Cochrane

      Editorial/indexed abstract · n/a

      Academic, Cochrane-affiliated.

      Limits: Not an independent study; restates the 4-RCT review.

    • Funding unknown

      Kox et al. 2014, PNAS

      RCT (acute experimental endotoxemia) · n=24 (12 trained, 12 control), healthy young men

      Radboud University; authors declared no conflict, but Wim Hof personally trained the participants.

      Limits: Tiny sample, healthy young men only, acute injected challenge, not a real disease; no longevity data.

    • Funding unknown

      Zwaag et al. 2022, Psychosom Med

      Mechanistic physiological pilot · Small pilot (tens of participants)

      Same Radboud group as Kox 2014.

      Limits: Mechanistic only; shows the effect is an adrenaline/alkalosis stress response, not disease treatment.

    • Independent

      Almahayni & Hammond 2024, PLOS One

      Systematic review of 8 trials (9 papers) · ~200-250 pooled, 15-48 per trial, ~86% male

      No specific funding; explicitly raised conflict-of-interest concerns about Hof's involvement.

      Limits: Very low quality evidence; 7/8 RCTs high bias concern; not generalizable; no disease or longevity outcomes.

    • Not applicable

      Wikipedia: deaths and scientific reception

      Documented adverse events and critical reception · Case/legal record; ~32 deaths allegedly linked by March 2024

      Aggregates Het Parool reporting, coroner findings, and the Metzger lawsuit.

      Limits: Journalistic and legal sources, not systematic surveillance; exact death count uncertain.

    • Not applicable

      Live Science: expert safety commentary

      Expert-sourced safety reporting · Expert commentary

      Science journalism quoting domain experts.

      Limits: Commentary, not a trial; used for the drowning and arrhythmia safety mechanism.

    • Independent

      Conboy et al., Nature 2005 (heterochronic parabiosis)

      Experimental animal study · Mice, young 2-3 mo and old 19-26 mo, surgically paired

      Academic labs; foundational basic science, no commercial product sponsor.

      Limits: Surgical mouse model; cannot be ethically or practically reproduced in humans, and is not an infusible product.

    • Independent

      Mehdipour et al., Aging 2020 (saline-albumin 'dilution' exchange)

      Animal study plus ex vivo human serum assay · Mice n=4 per cohort; serum from 4 older humans (65-70)

      NIH grants plus Open Philanthropy; authors declare no financial conflicts.

      Limits: Very small mouse sample; human evidence is only a preliminary cell-culture signal, not clinical outcomes.

    • Not applicable

      FDA safety statement, Feb 19 2019 (Gottlieb and Marks)

      Regulatory safety statement · N/A

      US federal regulator.

      Limits: A warning, not a trial; establishes absence of proven benefit and known risks rather than testing efficacy.

    • Industry-funded

      AMBAR primary results, Boada et al. 2020 (plasma exchange in Alzheimer's)

      Randomized, double-blind, sham-controlled trial · 347 randomized, mild/moderate Alzheimer's, 41 centers, ~14 months

      Sponsored by Grifols, maker of the albumin and IVIG tested; multiple authors are Grifols employees. Sponsor-favorable pattern.

      Limits: Did not meet both co-primary endpoints; in diagnosed Alzheimer's, not healthy aging, so cannot establish rejuvenation.

    • Industry-funded

      AMBAR design paper, Boada et al. 2019

      Clinical trial design/protocol paper · 347 randomized across 4 arms

      States verbatim the trial 'is sponsored by Grifols, a manufacturer of therapeutic human serum albumin and intravenous immune globulin.'

      Limits: Protocol paper only; reports design, not efficacy.

  • SupplementsNMN Unproven
    • Industry-funded

      Frontiers in Nutrition, 2022

      RCT · n=30 / 12 wk

      Mitsubishi Corp Life Sciences, funded; supplied the NMN; 4 author-employees.

      Limits: Small; surrogate endpoint (NAD+); single trial; healthy Japanese adults only.

    • Industry-funded

      npj Aging, 2022

      RCT · ~10/group / 6–12 wk

      Mitsubishi, funded; supplied NMN; 3 author-employees.

      Limits: Very small (22 dropped after a dosing error); gains only in gait speed + left grip; “nominally significant.”

    • Industry-funded

      Scientific Reports, 2022

      RCT (safety) · n=31 / 4 wk

      Mitsubishi, funded; manufactured NMN; 5 author-employees.

      Limits: Very short; high dose (1,250 mg); short-term safety only, not efficacy.

    • Independent

      Cureus, 2024

      Systematic review · pooled small/short RCTs

      Declares no funding and no conflicts.

      Limits: Improvement was non-significant; Cureus is lightly peer-reviewed.

    • Mixed funding

      J. Int. Soc. Sports Nutrition, 2021

      RCT · n=48 / 6 wk

      China government-funded, but NMN supplied by manufacturer GeneHarbor.

      Limits: Exercise co-intervention (confounder); VO₂max unchanged; multiple dose arms.

    • Independent

      Crit. Reviews in Food Science & Nutrition, 2024

      Meta-analysis (12 RCTs, 513) · strongest synthesis

      Declares no funding and no conflicts.

      Limits: No significant glucose/lipid benefit; authors warn benefits “may be exaggerated.”

    • Not applicable

      bioRxiv, 2024

      Animal, preprint · mice

      Not assessed (animal preprint).

      Limits: Preprint, not peer-reviewed; lifespan effect female-only; animal→human gap.

Each study here is appraised inside one of our checks, click any check to read the full evidence and sources. Funding reflects what we could verify; figures cover checks currently in review. Caveat is journalism, not medical advice, and takes no industry money. How we grade.